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Biochemistry, Biophysics, and Structural Biology Commons

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Retrospective Theses and Dissertations

Physical Sciences and Mathematics

2007

Articles 1 - 2 of 2

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Advanced Detection And Separation Methods: Developments In Surface-Enhanced Raman Scattering Readout Immunoassays And Electrochemically Modulated Liquid Chromatography, Betsy Jean Yakes Jan 2007

Advanced Detection And Separation Methods: Developments In Surface-Enhanced Raman Scattering Readout Immunoassays And Electrochemically Modulated Liquid Chromatography, Betsy Jean Yakes

Retrospective Theses and Dissertations

This dissertation explores the use of novel detection methods for biological and chemical components commonly found in the environment. It encompasses two techniques: surface-enhanced Raman scattering (SERS) and electrochemically modulated liquid chromatography (EMLC).;Immunoassays using SERS as a readout tool have been developed in this laboratory and have shown low levels of detection (i.e., pico- to femtomolar and single binding event detection) for disease and biowarfare agents. This thesis seeks to further the performance of this platform for bacteria detection and explore strategies to increase the SERS response. Specifically, the first section of this dissertation focuses on the detection ...


Investigation Of The Peptide-Lipid Interactions Of A Beta-Hairpin Antimicrobial Peptide Using Solid-State Nmr, Rajeswari Mani Jan 2007

Investigation Of The Peptide-Lipid Interactions Of A Beta-Hairpin Antimicrobial Peptide Using Solid-State Nmr, Rajeswari Mani

Retrospective Theses and Dissertations

Solid-state NMR (SSNMR) has gained importance as a versatile tool to elucidate the structure and function of many chemical and biological molecules. In this thesis, we have used SSNMR to study the interactions of an antimicrobial peptide (AMP), Protegrin-1 (PG-1) with membranes of varying lipid composition. Based on our studies we have proposed a model for the mechanism of action of PG-1 with two different lipid membranes. PG-1 is a disulfide-linked beta-sheet peptide with broad-spectrum antimicrobial activities. Similar to many AMPs, it selectively disrupts the anionic membranes of microbial cells but leaves the cholesterol-rich zwitterionic mammalian cell membranes intact.;Studying ...