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Biochemistry, Biophysics, and Structural Biology Commons

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Retrospective Theses and Dissertations

Physical Sciences and Mathematics

2002

Articles 1 - 2 of 2

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Nonphotochemical Hole-Burning Imaging Studies Of In Vitro Carcinoma And Normal Cells Utilizing A Mitochondrial Specific Dye , Richard Joseph Walsh Jan 2002

Nonphotochemical Hole-Burning Imaging Studies Of In Vitro Carcinoma And Normal Cells Utilizing A Mitochondrial Specific Dye , Richard Joseph Walsh

Retrospective Theses and Dissertations

Low temperature Nonphotochemical Hole Burning (NPHB) Spectroscopy of the dye rhodamine 800 (MF680) was applied for the purpose of discerning differences between cultured normal and carcinoma ovarian surface epithelial (OSE) cells. Both the cell lines were developed and characterized at the Mayo Clinic (Rochester, MN), with the normal cell line having been transfected with a strain of temperature sensitive Simian Virus 40 Large T Antigen (SV40) for the purpose of extending the life of the cell culture without inducing permanent changes in the characteristics of the cell line. The cationic lipophilic fluorophore rhodamine 800 preferentially locates in in situ mitochondria ...


Complexes Of Palladium(Ii) And Platinum(Ii) As Synthetic Proteases , Nebojša M. Milović Jan 2002

Complexes Of Palladium(Ii) And Platinum(Ii) As Synthetic Proteases , Nebojša M. Milović

Retrospective Theses and Dissertations

Selective cleavage of proteins is a common procedure in many biochemical applications ranging from standard protein sequencing to novel methods in proteomics and bioengineering. Few enzymes and synthetic reagents are available for this important task, but new chemical reagents with improved efficiency and adjustable selectivity are highly desired. Complexes of palladium(II) and platinum(II), two chemically similar transition-metal ions, are new reagents for selective cleavage of peptides and proteins. The cleavage by Pd(II) complexes, such as [Pd(H2O)4]2+ or cis-[Pd(en)(H 2O)2]2+, consistently occurs in weakly acidic aqueous solutions at the amide ...