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Biochemistry, Biophysics, and Structural Biology Commons

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Articles 1 - 4 of 4

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Characterization Of Biomacromolecules By Nuclear Magnetic Resonance Spectroscopy , Wayne Robert Baker Jan 1996

Characterization Of Biomacromolecules By Nuclear Magnetic Resonance Spectroscopy , Wayne Robert Baker

Retrospective Theses and Dissertations

Two proteins were examined by Nuclear Magnetic Resonance (NMR) spectroscopy. The Emb-1 protein of Daucus carota was found to have few spectral characteristics indicative of a stable tertiary structure. In particular, line-widths were narrow, chemical shift dispersion was poor and nuclear Overhauser effect spectra were sparse. The NMR spectra of Ribonuclease A was examined at a range of pH values and the chemical shifts of a selected number of resonances was plotted as a function of the pH. From these titrations curves pKa values were determined and assigned to specific ionizing groups within the protein based, in part, on physical ...


Single Cell Analysis By Capillary Electrophoresis With Laser-Induced Native Fluorescence Detection , Sheri Joanne Lillard Jan 1996

Single Cell Analysis By Capillary Electrophoresis With Laser-Induced Native Fluorescence Detection , Sheri Joanne Lillard

Retrospective Theses and Dissertations

Individual mammalian cells were analyzed by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). This technique was chosen due to its high separation efficiencies, small sample volumes and sensitive detection. Native fluorescence was used, in which the analyte was not tagged with a fluorophore. 275-nm excitation gave attomole (amol = 10-18 mol) detection limits for the intracellular species of interest. Two projects are described in which hemoglobin (Hb) variants were determined in single red blood cells. In the third experiment, individual mast cells were degranulated on-column, and exocytosis and serotonin release were monitored temporally;First, single red blood cells, in which Hb ...


Bacterial Physiology And Enzymology Of The Membrane-Associated Methane Monooxygenase And Ammonia Oxidation System From Methylococcus Capsulatus Bath, 1992-1996 , James Allan Zahn Jan 1996

Bacterial Physiology And Enzymology Of The Membrane-Associated Methane Monooxygenase And Ammonia Oxidation System From Methylococcus Capsulatus Bath, 1992-1996 , James Allan Zahn

Retrospective Theses and Dissertations

The enzymology and microbial physiology of methane and ammonia oxidation by the membrane-associated methane monooxygenase (pMMO) from the obligate methylotroph, Methylococcus capsulatus Bath were studied. Conditions leading to the stabilization of the pMMO were defined, and an active preparation of the pMMO from M. capsulatus Bath was isolated by ion exchange and hydrophobic interaction chromatography using dodecyl-[beta]-D-maltoside as the detergent. The active preparation consisted of three major polypeptides with molecular masses of 47,000 and 27,000, and 25,000 Da and contained 2.5 iron and 14.5 copper atoms per mole of enzyme. The 27,000 ...


Applications Of Partial Filling Micellar Electrokinetic Chromatography: From Electrospray Mass Spectrometry To Fluorescence Polarization Immunodetection , Wendy Marie Nelson Jan 1996

Applications Of Partial Filling Micellar Electrokinetic Chromatography: From Electrospray Mass Spectrometry To Fluorescence Polarization Immunodetection , Wendy Marie Nelson

Retrospective Theses and Dissertations

Mechanistic studies of partial filling micellar electrokinetic chromatography PF-MEKC) and a theoretical model for the prediction of separation behavior in PF-MEKC was developed in this work. Along with the theoretical development of PF-MEKC, two different detection modes were applied to PF-MEKC, in contrast to the traditional UV detection used in micellar electrokinetic chromatography (MEKC). The first detection mode coupled PF-MEKC with eletrospray ionization mass spectrometry. The second detection mode developed in this work is PF-MEKC coupled with fluorescence polarization immunodetection;First, in the mechanistic studies of PF-MEKC a theoretical model was proposed for predicting the separation behavior of triazine herbicides ...