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Biochemistry, Biophysics, and Structural Biology Commons

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Retrospective Theses and Dissertations

Physical Sciences and Mathematics

1995

Articles 1 - 4 of 4

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Effects Of Ear Developmental Temperature On Fine Structure Of Maize Starch , Ting-Jang Lu Jan 1995

Effects Of Ear Developmental Temperature On Fine Structure Of Maize Starch , Ting-Jang Lu

Retrospective Theses and Dissertations

Two genetically unrelated normal dent maize inbreds, ICI63 and ICI92, with different heterotic backgrounds were used for studying environmental temperature effects on grain yield and quality. These two maize inbreds are used in commercially available hybrids and are proprietary to ICI Seeds. The maize inbreds were grown in a greenhouse to control the growing condition of the plants. After 14 days post-pollination, the ears were wrapped in temperature control devices set at 25 or 35∘ C during the grain-filling period. Grain yield, kernel weight, and kernel density were less for ears developing at 35∘ C than for those at 25 ...


Spectral Hole Burning Studies Of Photosystem Ii , Hai-Chou Chang Jan 1995

Spectral Hole Burning Studies Of Photosystem Ii , Hai-Chou Chang

Retrospective Theses and Dissertations

Low temperature absorption and hole burning spectroscopies were applied to the D1-D2-cyt b[subscript]559, and the CP47 and CP43 antenna protein complexes of Photosystem II from higher plants. Low temperature transient and persistent hole-burning data and theoretical calculations on the kinetics and temperature dependence of the P680 hole profile are presented and provide convincing support for the linker model. Implicit in the linker model is that the 684-nm-absorbing Chl a serve to shuttle energy from the proximal antenna complex to reaction center. The stoichiometry of isolated Photosystem II Reaction Center (PSII RC) in several different preparations is also discussed ...


Photophysics And Activity Of Biological Systems Containing The Optical Probes 7-Azatryptophan And Its Analogs , Rebecca Lynne Rich Jan 1995

Photophysics And Activity Of Biological Systems Containing The Optical Probes 7-Azatryptophan And Its Analogs , Rebecca Lynne Rich

Retrospective Theses and Dissertations

A major use of photophysical apparatus is the study of biological systems, for example, peptides, proteins, and DNA, that contain one or more chromophores. In recent years the incorporation of nonnatural fluorescent compounds into these systems has gained great popularity. A number of optical 'tags' have been suggested as detection tools of structure and function; we propose the use of the tryptophan derivative, 7-azatryptophan, as an intrinsic probe in photophysical analyses of proteins. Not only is this chromophore spectroscopically distinct from tryptophan and all other natural amino acids, but we have demonstrated the successful incorporation of 7-azatryptophan into peptide sequences ...


Line Narrowing Spectroscopic Studies Of Dna-Carcinogen Adducts And Dna-Dye Complexes , Myungkoo Suh Jan 1995

Line Narrowing Spectroscopic Studies Of Dna-Carcinogen Adducts And Dna-Dye Complexes , Myungkoo Suh

Retrospective Theses and Dissertations

Laser-induced fluorescence line narrowing (FLN) and non-line narrowing (NLN) spectroscopic methods were applied to conformational studies of stable DNA adducts of the 7[beta],8[alpha]-dihydoxy-9[alpha],10[alpha]-epoxy-7,8,9, 10-tetrahydrobenzo (a) pyrene (anti-BPDE). Stereochemically distinct (+)-trans-, (-)-trans-, (+)-cis- and (-)-cis adducts of anti-BPDE bound to exocyclic amino group of the central guanine in the oligonucleotide, 5'-d(CCATCGCTACC) · (GGTAGCGATGG), exist in a mixture of conformations in frozen aqueous buffer matrices. The (+)-trans adduct adopts primarily an external conformation with a smaller fraction (~25%) exists in a partially base-stacked conformation. Both cis adducts were found to be ...