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Biochemistry, Biophysics, and Structural Biology Commons

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Retrospective Theses and Dissertations

Physical Sciences and Mathematics

1991

Articles 1 - 2 of 2

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Selective Labeling Of Peptides, Metalloproteins, And Proteolytic Enzymes With Pt(Ii) Complexes And Electron-Transfer Reactions Of Native And Cross-Linked Metalloproteins , Herb M. Brothers Ii Jan 1991

Selective Labeling Of Peptides, Metalloproteins, And Proteolytic Enzymes With Pt(Ii) Complexes And Electron-Transfer Reactions Of Native And Cross-Linked Metalloproteins , Herb M. Brothers Ii

Retrospective Theses and Dissertations

The transition-metal complex (Pt(trpy)Cl) [superscript]+ selectively labels the amino acids Cys and His, but is unreactive toward Met. The (Pt(trpy)Cl) [superscript]+ complex also forms complexes with nonbiological ligands to form novel compounds with metal-metal or [pi]-[pi] interactions;The homologous gold reagent, (Au(trpy)Cl) [superscript]2+ reacts with Trp, Cys, His, and Met. The oxidative properties of the gold complex makes it an invasive reagent toward proteins, however;In reactions with proteins, the complex (Pt(trpy)Cl) [superscript]+ exhibits unexpected selectivity toward amino acid side chains in cytochromes c. His residues are labeled in greater ...


Study Of Secondary Structure Of Protein Sequences By Linear Algebra , Wei-Hua Hsieh Jan 1991

Study Of Secondary Structure Of Protein Sequences By Linear Algebra , Wei-Hua Hsieh

Retrospective Theses and Dissertations

Using encoding schemes, we study the relation between amino acids and protein structure in linear spaces;Local protein-structure prediction schemes use the amino acid sequences in a short subchain of a protein to predict the protein secondary structure of the middle residue of the chain;Two relatively reliable, objective and quantitative local prediction schemes are Robson et al.'s information theory method and and Qian & Sejnowski's neural network models. The latter achieved 64% accuracy for three-state predictions in 1989, the best prediction performance yet recorded;We assign to the 20 amino acids, through a one-to-one correspondence, the 20 columns (unit vectors) of the 20 x 20 identity matrix. Then any chain of k amino acids may be represented as a ...