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Biochemistry, Biophysics, and Structural Biology Commons

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Retrospective Theses and Dissertations

Microbiology

1950

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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Mechanism Of Ammoniation In Aerobacter Aerogenes , Eric Beaumont Fowler Jan 1950

Mechanism Of Ammoniation In Aerobacter Aerogenes , Eric Beaumont Fowler

Retrospective Theses and Dissertations

Whole cells and cell-free extracts of Aerobacter aerogenes possess an enzyme system which actively ammoniates alpha-ketoglutarate The enzyme was obtained in cell-free solution; its action was similar to that in resting cells or growing cells;The concentration of glutamate formed by growing cells in the aerobic dissimilation of a carbon source in higher than that formed under anaerobic conditions. The same is not true far long dialyzed juices where more glutamate is formed anaerobically;The enzyme(s) participating in the ammoniation of alpha-ketoglutarate may be located near the surface of the cell;Short time experiments employing N15 have shown aspartate ...