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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Structure And Function Analysis Of Lin-14, A Temporal Regulator Of Postembryonic Developmental Events In Caenorhabditis Elegans, Yang Hong, Rosalind C. Lee, Victor Ambros Dec 1999

Structure And Function Analysis Of Lin-14, A Temporal Regulator Of Postembryonic Developmental Events In Caenorhabditis Elegans, Yang Hong, Rosalind C. Lee, Victor Ambros

Open Dartmouth: Faculty Open Access Scholarship

During postembryonic development of Caenorhabditis elegans, the heterochronic gene lin-14 controls the timing of developmental events in diverse cell types. Three alternativelin-14 transcripts are predicted to encode isoforms of a novel nuclear protein that differ in their amino-terminal domains. In this paper, we report that the alternative amino-terminal domains of LIN-14 are dispensable and that a carboxy-terminal region within exons 9 to 13 is necessary and sufficient for in vivo LIN-14 function. A transgene capable of expressing only one of the three alternativelin-14 gene products rescues a lin-14 null mutation and is developmentally regulated by lin-4. This shows ...


Sec34p, A Protein Required For Vesicle Tethering To The Yeast Golgi Apparatus, Is In A Complex With Sec35p, Susan M. Vanrheenen, Xiaochun Cao, Stephanie K. Sapperstein, Elbert C. Chiang, Vladimir V. Lupashin, Charles Barlowe, M. Gerard Waters Nov 1999

Sec34p, A Protein Required For Vesicle Tethering To The Yeast Golgi Apparatus, Is In A Complex With Sec35p, Susan M. Vanrheenen, Xiaochun Cao, Stephanie K. Sapperstein, Elbert C. Chiang, Vladimir V. Lupashin, Charles Barlowe, M. Gerard Waters

Open Dartmouth: Faculty Open Access Scholarship

A screen for mutants of Saccharomyces cerevisiae secretory pathway components previously yielded sec34, a mutant that accumulates numerous vesicles and fails to transport proteins from the ER to the Golgi complex at the restrictive temperature (Wuestehube, L.J., R. Duden, A. Eun, S. Hamamoto, P. Korn, R. Ram, and R. Schekman. 1996. Genetics. 142:393–406). We find that SEC34 encodes a novel protein of 93-kD, peripherally associated with membranes. The temperature-sensitive phenotype of sec34-2 is suppressed by the rab GTPase Ypt1p that functions early in the secretory pathway, or by the dominant form of the ER to Golgi complex ...


Cbfa2 Is Required For The Formation Of Intra-Aortic Hematopoietic Clusters, Trista North, Ting-Lei Gu, Stacy Terryl, Qing Wang, Louisa Howard, Michael Binder, Miguel Marín-Padilla, Nancy A. Speck May 1999

Cbfa2 Is Required For The Formation Of Intra-Aortic Hematopoietic Clusters, Trista North, Ting-Lei Gu, Stacy Terryl, Qing Wang, Louisa Howard, Michael Binder, Miguel Marín-Padilla, Nancy A. Speck

Open Dartmouth: Faculty Open Access Scholarship

Cbfa2 (AML1) encodes the DNA-binding subunit of a transcription factor in the small family of core-binding factors (CBFs). Cbfa2 is required for the differentiation of all definitive hematopoietic cells, but not for primitive erythropoiesis. Here we show that Cbfa2 is expressed in definitive hematopoietic progenitor cells, and in endothelial cells in sites from which these hematopoietic cells are thought to emerge. Endothelial cells expressing Cbfa2 are in the yolk sac, the vitelline and umbilical arteries, and in the ventral aspect of the dorsal aorta in the aorta/genital ridge/mesonephros (AGM) region. Endothelial cells lining the dorsal aspect of the ...


A Role For The Light-Dependent Phosphorylation Of Visual Arrestin, Paul G. Alloway, Patrick J. Dolph May 1999

A Role For The Light-Dependent Phosphorylation Of Visual Arrestin, Paul G. Alloway, Patrick J. Dolph

Open Dartmouth: Faculty Open Access Scholarship

Arrestins are regulatory proteins that participate in the termination of G protein-mediated signal transduction. The major arrestin in the Drosophila visual system, Arrestin 2 (Arr2), is phosphorylated in a light-dependent manner by a Ca2+/calmodulin-dependent protein kinase and has been shown to be essential for the termination of the visual signaling cascade in vivo. Here, we report the isolation of nine alleles of the Drosophila photoreceptor cell-specific arr2 gene. Flies carrying each of these alleles underwent light-dependent retinal degeneration and displayed electrophysiological defects typical of previously identified arrestin mutants, including an allele encoding a protein that lacks the major Ca2 ...