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Chemistry Faculty Publications

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Articles 61 - 90 of 159

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

In Vivo Characterization Of The Integrin Beta(3) As A Receptor For Hantaan Virus Cellular Entry, Jin-Won Song, Ki-Joon Song, Luck-Ju Baek, Blaise Frost Apr 2005

In Vivo Characterization Of The Integrin Beta(3) As A Receptor For Hantaan Virus Cellular Entry, Jin-Won Song, Ki-Joon Song, Luck-Ju Baek, Blaise Frost

Chemistry Faculty Publications

No abstract provided.


Correction Of The Literature Citing Monoiodo-8-Quinolinols: A Critical Review / Herman Gershon And Donald D. Clarke Harding Laboratory, The New York Botanical Garde, And Fordham University, Bronx, New York 10458, Usa, Herman Gershon, Donald Dudley Clarke Phd, Donald Dudley Clarke Phd Jan 2005

Correction Of The Literature Citing Monoiodo-8-Quinolinols: A Critical Review / Herman Gershon And Donald D. Clarke Harding Laboratory, The New York Botanical Garde, And Fordham University, Bronx, New York 10458, Usa, Herman Gershon, Donald Dudley Clarke Phd, Donald Dudley Clarke Phd

Chemistry Faculty Publications

Until 1995, all papers that claimed preparation or further study with monoiodo-8-quinolinol were really working with the 7-iodo isomer. Of the 83 relevant citations 67 dealt with incorrect structural assignments, 13 were critiqued due to errors made in synthesis and interpretation, and 9 had the correct structural assignments. This literature review made the following conclusions apparent. 1. Prototropic forms of 8- quinolinol influence orientation of electrophiles. 2. Under strong acidic conditions, the 5 position is favored. 3. Under mild acidic conditions, mixtures of 5 and 7 substituted compounds are formed. 4. Under basic conditions, the incoming electrophile favors the 7 ...


Evaluating The Contributions Of Desolvation And Base-Stacking During Translesion Dna Synthesis, Xuemei Zhang, Irene Lee, Anthony J. Berdis May 2004

Evaluating The Contributions Of Desolvation And Base-Stacking During Translesion Dna Synthesis, Xuemei Zhang, Irene Lee, Anthony J. Berdis

Chemistry Faculty Publications

DNA polymerases catalyze the insertion of a nucleoside triphosphate into the growing polymer chain using the template strand as a guide. Numerous factors such as hydrogen bonding interactions, base-stacking contributions, and desolvation play important roles in controlling the efficiency and fidelity of this process. We previously demonstrated that 5-nitro-indolyl-2′-deoxyriboside triphosphate, a non-natural nucleobase with enhanced base-stacking properties, was more efficiently inserted opposite a non-templating DNA lesion compared to natural templating nucleobases (E. Z. Reineks and A. J. Berdis, Biochemistry, 2004, 43, 393–404). The catalytic enhancement was proposed to reflect increased base-stacking interactions of the non-natural nucleobase with the ...


Synergistic Mixtures Of Fungitoxic Monochloro And Dichloro-8-Quinolinols Against Five Fungi / Herman Gershon, Muriel Gershon, & Donald D. Clarke Harding Laboratory, The New York Botanical Garden, Bronx, Ny 10458-5126, Usa; Department Of Chemistry, Fordham University, Bronx, Ny 10458-9993, Usa, Herman Gershon, Muriel Gershon, Donald Dudley Clarke Phd Jan 2004

Synergistic Mixtures Of Fungitoxic Monochloro And Dichloro-8-Quinolinols Against Five Fungi / Herman Gershon, Muriel Gershon, & Donald D. Clarke Harding Laboratory, The New York Botanical Garden, Bronx, Ny 10458-5126, Usa; Department Of Chemistry, Fordham University, Bronx, Ny 10458-9993, Usa, Herman Gershon, Muriel Gershon, Donald Dudley Clarke Phd

Chemistry Faculty Publications

Fourteen mono- and dichloro-8-quinolinols were tested against five fungi (Aspergillus niger, A. oryzae, Myrothecium verrucaria, Trichoderma viride, and Mucor circinelloides) and compared with the fungitoxicity of 8- quinolinol in Yeast Nitrogen Base containing l% D-glucose and 0.088% L-asparagine. All of the compounds were more fungitoxic than 8-quinolinol except for the surprising activity of 8-quinolinol against A. oryzae. Mixtures of the MICs of monochloro- and dichloro-8-quinolinols in which the halogens were in different positions of the quinoline ring showed synergism. Comparable mixtures in which one position of each compound was occupied by the same halogen showed additive activity. In a ...


Evaluating The Effects Of Enhanced Processivity And Metal Ions On Translesion Dna Replication Catalyzed By The Bacteriophage T4 Dna Polymerase, Edmunds Z. Reineks, Anthony J. Berdis May 2003

Evaluating The Effects Of Enhanced Processivity And Metal Ions On Translesion Dna Replication Catalyzed By The Bacteriophage T4 Dna Polymerase, Edmunds Z. Reineks, Anthony J. Berdis

Chemistry Faculty Publications

The fidelity of DNA replication is achieved in a multiplicative process encompassing nucleobase selection and insertion, removal of misinserted nucleotides by exonuclease activity, and enzyme dissociation from primer/templates that are misaligned due to mispairing. In this study, we have evaluated the effect of altering these kinetic processes on the dynamics of translesion DNA replication using the bacteriophage T4 replication apparatus as a model system. The effect of enhancing the processivity of the T4 DNA polymerase, gp43, on translesion DNA replication was evaluated using a defined in vitro assay system. While the T4 replicase (gp43 in complex with gp45) can ...


Examination Of The Role Of The Clamp-Loader And Atp Hydrolysis In The Formation Of The Bacteriophage T4 Polymerase Holoenzyme, Michael A. Trakselis, Anthony J. Berdis, Stephen J. Benkovic Feb 2003

Examination Of The Role Of The Clamp-Loader And Atp Hydrolysis In The Formation Of The Bacteriophage T4 Polymerase Holoenzyme, Michael A. Trakselis, Anthony J. Berdis, Stephen J. Benkovic

Chemistry Faculty Publications

Transient kinetic analyses further support the role of the clamp-loader in bacteriophage T4 as a catalyst which loads the clamp onto DNA through the sequential hydrolysis of two molecules of ATP before and after addition of DNA. Additional rapid-quench and pulse-chase experiments have documented this stoichiometry. The events of ATP hydrolysis have been related to the opening/closing of the clamp protein through fluorescence resonance energy transfer (FRET). In the absence of a hydrolysable form of ATP, the distance across the subunit interface of the clamp does not increase as measured by intramolecular FRET, suggesting gp45 cannot be loaded onto ...


Effect Of Dimethyl Sulfoxide And Dimethylformamide On The Stability Of 4-Halo-8quinolinols / Herman Gershon, Donald D. Clarke, And John J. Mcmahon Department Of Chemistry, Fordham University, Bronx, Ny 10458-9993, Usa New York Botanical Garden, Bronx, Ny 10458-9993, Usa, Herman Gershon, Donald Dudley Clarke Phd, John J. Mcmahon Jan 2003

Effect Of Dimethyl Sulfoxide And Dimethylformamide On The Stability Of 4-Halo-8quinolinols / Herman Gershon, Donald D. Clarke, And John J. Mcmahon Department Of Chemistry, Fordham University, Bronx, Ny 10458-9993, Usa New York Botanical Garden, Bronx, Ny 10458-9993, Usa, Herman Gershon, Donald Dudley Clarke Phd, John J. Mcmahon

Chemistry Faculty Publications

Polyhalo-8-quinolinols with chlorine or bromine in position 4 were not stable in DMSO or DMF. The degradation product from 4,5-dichloro-8-quinolinol was 5-chloro-4,8-quinolindiol and the major product from 4,5-dibromo-8-quinolinol was 3,5-dibromo-4,8-quinolindiol. 4,5,7-Trichloro- and 4,5,7-tribromo-8-quinolinols yielded similar hydrolytic products, and for the bromo compound, a rebrominated product in DMSO. In DMF rebrornination did not occur. In pyridine-d5 these reactions did not take place, indicating a special ability of DMSO and DMF to cause such hydrolysis at position 4 of 4-halo-8-quinolinols


Urer, The Transcriptional Activator Of The Proteus Mirabilis Urease Gene Cluster, Is Required For Urease Activity And Virulence In Experimental Urinary Tract Infections, Jonathan D. Dattelbaum, C. Virginia Lockatell, David E. Johnson, Harry L.T. Mobley Jan 2003

Urer, The Transcriptional Activator Of The Proteus Mirabilis Urease Gene Cluster, Is Required For Urease Activity And Virulence In Experimental Urinary Tract Infections, Jonathan D. Dattelbaum, C. Virginia Lockatell, David E. Johnson, Harry L.T. Mobley

Chemistry Faculty Publications

Proteus mirabilis, a cause of complicated urinary tract infection, produces urease, an essential virulence factor for this species. UreR, a member of the AraC/XylS family of transcriptional regulators, positively activates expression of the ure gene cluster in the presence of urea. To specifically evaluate the contribution of UreR to urease activity and virulence in the urinary tract, a ureR mutation was introduced into P. mirabilis HI4320 by homologous recombination. The isogenic ureR::aphA mutant, deficient in UreR production, lacked measurable urease activity. Expression was not detected in the UreR-deficient strain by Western blotting with monoclonal antibodies raised against UreD ...


A Comparison Of The Low Mode And Monte Carlo Conformational Search Methods, Carol A. Parish, Rosina Lombardi, Kent Sinclair, Emelyn Smith, Alla Goldberg, Melissa Rappleye, Myrianne Dure Oct 2002

A Comparison Of The Low Mode And Monte Carlo Conformational Search Methods, Carol A. Parish, Rosina Lombardi, Kent Sinclair, Emelyn Smith, Alla Goldberg, Melissa Rappleye, Myrianne Dure

Chemistry Faculty Publications

The Low Mode (LM) and Monte Carlo (MC) conformational search methods were compared on three diverse molecular systems; (4R, 5S, 6S, 7R)-hexahydro-5,6-dihydroxy-1,3,4,7-tetrakis(phenylmethyl)-2H-1,3-diazapin-2-one (1), 2-methoxy-2-phenyl-2-triflouromethyl-N-α-methyl benzyl propanamide (2) and a trimeric 39-membered polyazamacrolide (3). We find that either method, or a combination of the methods, is equally efficient at searching the conformational space of the smaller molecular systems while a 50:50 hybrid of Low Mode and Monte Carlo is most efficient at searching the space of the larger molecular system.


Preparation Of 6-Fluoro-8-Quinolinol And Related 6-Fluoroquinolines / Herman Gershon, Donald D. Clarke, And Muriel Gershon Department Of Chemistry, Fordham University, Bronx, New York 10458 U.S.A., New York Botanical Garden, Bronx, New York 10458, U.S.A., Herman Gershon, Donald Dudley Clarke Phd, Muriel Gershon Jan 2002

Preparation Of 6-Fluoro-8-Quinolinol And Related 6-Fluoroquinolines / Herman Gershon, Donald D. Clarke, And Muriel Gershon Department Of Chemistry, Fordham University, Bronx, New York 10458 U.S.A., New York Botanical Garden, Bronx, New York 10458, U.S.A., Herman Gershon, Donald Dudley Clarke Phd, Muriel Gershon

Chemistry Faculty Publications

6-Fluoro-8-quinolinol was prepared from 2-amino-5-fluorophenol by a Skraup synthesis. No synergism was observed between 5-fluoro- and 6-fluoro-8-quinolinols or between 6-fluoro-8-quinolinolsuoro- and 7-fluoro-8-quinolinols against any of the six fungi in our test system (Aspergillus niger, A. oryzae, Myrothecium verrucaria, Trichoderma viride, Mucor cirinelloides, and Trichophyton mentagrophytes) in Sabouraud dextrose broth. Unlike the fluoro-8-quinolinols, the 8-quinolinols comparably substituted with chlorine or bromine did form synergistic mixtures. This is attributed to steric factors


Preparation And Antifungal Activity Of 3-Iodo- And 6-Iodo-8-Quinolinols / Herman Gershon, Donald D. Clarke, John J. Mcmahon, And Muriel Gershon Department Of Chemistry, Fordham University, Bronx, Ny 10458-9993, Usa New York Botanical Garden, Bronx, Ny 10458-9993, Usa, Herman Gershon, Donald Dudley Clarke Phd, John J. Mcmahon, Muriel Gershon Jan 2002

Preparation And Antifungal Activity Of 3-Iodo- And 6-Iodo-8-Quinolinols / Herman Gershon, Donald D. Clarke, John J. Mcmahon, And Muriel Gershon Department Of Chemistry, Fordham University, Bronx, Ny 10458-9993, Usa New York Botanical Garden, Bronx, Ny 10458-9993, Usa, Herman Gershon, Donald Dudley Clarke Phd, John J. Mcmahon, Muriel Gershon

Chemistry Faculty Publications

3-Iodo- and 6-Iodo-8-quinolinols were prepared and tested against six fungi: Aspergillus niger, A. oryzae, Myrothecium verrucaria, Trichoderma viride, Mucor cirinelloides, and Trichophyton mentagrophytes in Sabouraud dextrose broth. A comparison with the previously known 5-iodo- and 7-iodo-8-quinolinols showed that the 6-iodo isomer was the most active


Identification Of The Domains Of Urer, An Arac-Like Transcriptional Regulator Of The Urease Gene Cluster In Proteus Mirabilis, Carrie A. Poore, Christopher Coker, Jonathan D. Dattelbaum, Harry L.T. Mobley Jan 2001

Identification Of The Domains Of Urer, An Arac-Like Transcriptional Regulator Of The Urease Gene Cluster In Proteus Mirabilis, Carrie A. Poore, Christopher Coker, Jonathan D. Dattelbaum, Harry L.T. Mobley

Chemistry Faculty Publications

Proteus mirabilis urease catalyzes the hydrolysis of urea to CO2 and NH3, resulting in urinary stone formation in individuals with complicated urinary tract infections. UreR, a member of the AraC family, activates transcription of the genes encoding urease enzyme subunits and accessory proteins, ureDABCEFG, as well as its own transcription in the presence of urea. Based on sequence homology with AraC, we hypothesized that UreR contains both a dimerization domain and a DNA-binding domain. A translational fusion of the leucine zipper dimerization domain (amino acids 302 to 350) of C/EBP and the C-terminal half of UreR (amino ...


Antifungal Activity Of Substituted 8-Quinolinol-5- And 7-Sulfonic Acids: A Mechanism Of Action Is Suggested Based On Intramolecular Synergism / Hermon Gershon, Muriel Gershon, & Donald D. Clarke Harding Laboratory, The New York Botanical Garden, Bronx, New York 10458, Usa; Department Of Chemistry, Fordham University, Bronx, New York 10458, Usa, Herman Gershon, Muriel Gershon, Donald Dudley Clarke Phd Jan 2001

Antifungal Activity Of Substituted 8-Quinolinol-5- And 7-Sulfonic Acids: A Mechanism Of Action Is Suggested Based On Intramolecular Synergism / Hermon Gershon, Muriel Gershon, & Donald D. Clarke Harding Laboratory, The New York Botanical Garden, Bronx, New York 10458, Usa; Department Of Chemistry, Fordham University, Bronx, New York 10458, Usa, Herman Gershon, Muriel Gershon, Donald Dudley Clarke Phd

Chemistry Faculty Publications

8-Quinolinol-5-sulfonic acid was nearly devoid of antimicrobial activity, due to what was believed to be an unfavorable partition coefficient. Since twenty six 8-quinolinol-5- and 7 -sulfonic acids were available from our previous work, they were tested against six fungi. The 7 -chloro and 7 -bromo-5-sulfonic acids and the 5-chloro and 5-bromo-7 -sulfonic acids showed fungal inhibition within one order of magnitude of that of 8-quinolinol. It is suggested that a nonchelating mechanism is in part responsible for this fungitoxicity. Five additional 5-sulfonic acids with chlorine in positions 3-, 6-, 3,6-, 3,7-, and 6,7- that were suitable for ...


Preparation And Fungitoxicity Of Some Trichloro-, Tribromo-, Tetrachloro-, And Tetrabromo-8-Quinolinols / Herman Gershon, Donald D. Clarke, And Muriel Gershon Department Of Chemistry, Fordham University, Bronx, New York 10458-9993 Usa, New York Botanical Garden, Bronx, New York 10458-9993 Usa, Herman Gershon, Donald Dudley Clarke Phd, Muriel Gershon Jan 2001

Preparation And Fungitoxicity Of Some Trichloro-, Tribromo-, Tetrachloro-, And Tetrabromo-8-Quinolinols / Herman Gershon, Donald D. Clarke, And Muriel Gershon Department Of Chemistry, Fordham University, Bronx, New York 10458-9993 Usa, New York Botanical Garden, Bronx, New York 10458-9993 Usa, Herman Gershon, Donald Dudley Clarke Phd, Muriel Gershon

Chemistry Faculty Publications

3,5,6-, 3,5,7-, 4,5,7-, and 5,6,7-trichloro- and -tribromo-8-quinolinols as well as 3,5,6,7- tetrachloro- and -tetrabromo-8-quinolinols were prepared and tested against six fungi (Aspergillus niger, Aspergillus oryzae, Myrothecium verrucaria, Trichoderma viride, Mucor cirinelloides, and Trichophyton mentagrophytes) in Sabouraud dextrose broth. The compounds strongly inhibit five fungi but not M. cirinelloides. They are less active than the related dichloro-8-quinolinols which is attributed to steric hindrance


Crystal Structure Of Bis(7-Nitro-8-Quinolinolato)Copper(Ii), Cuc18h10o6n4 / M. Shoja, H. Gershon And D. D. Clarke Fordham University, Department Of Chemistry, Fordham Road, Bronx, Ny 10458, Usa, Massud Shoja, Herman Gershon, Donald Dudley Clarke Phd Jan 2000

Crystal Structure Of Bis(7-Nitro-8-Quinolinolato)Copper(Ii), Cuc18h10o6n4 / M. Shoja, H. Gershon And D. D. Clarke Fordham University, Department Of Chemistry, Fordham Road, Bronx, Ny 10458, Usa, Massud Shoja, Herman Gershon, Donald Dudley Clarke Phd

Chemistry Faculty Publications

C1sHwCuN406, monoclinic, P121/cl (No. 14), a= 4.567(9) Å, b = 10.723(8) Å, c = 17.095(6) Å, ~ = 100.0(1)0 , v = 824.5 Å3 , Z = 2, Rgt(F) = 0.043, wRgt(F) = 0.067, T = 293 K


Synthesis Of 2-Acetamido-5,6-Dihalophenyl Acetates / Donald D. Clarke, Herman Gershon, And John J. Mcmahon Department Of Chemistry, Fordham University, Bronx, Ny 10458, Usa, Donald Dudley Clarke Phd, Herman Gershon, John J. Mcmahon Jan 2000

Synthesis Of 2-Acetamido-5,6-Dihalophenyl Acetates / Donald D. Clarke, Herman Gershon, And John J. Mcmahon Department Of Chemistry, Fordham University, Bronx, Ny 10458, Usa, Donald Dudley Clarke Phd, Herman Gershon, John J. Mcmahon

Chemistry Faculty Publications

2-Acetamido-5,6-dihalophenyl acetates were synthesized as intermediates for the preparation of 6, 7-dihalo-8-quinolinols via the Skraup procedure


Relationship Between Udp-Glucose 4-Epimerase Activity And Oligoglucose Glycoforms In Two Strains Of Neisseria Meningitidis, F K. Lee, B W. Gibson, William Melaugh, A Zaleski, M A. Apicella Jan 1999

Relationship Between Udp-Glucose 4-Epimerase Activity And Oligoglucose Glycoforms In Two Strains Of Neisseria Meningitidis, F K. Lee, B W. Gibson, William Melaugh, A Zaleski, M A. Apicella

Chemistry Faculty Publications

Sodium dodecyl sulfate-polyacrylamide gel analysis of lipooligosaccharide (LOS) from Neisseria meningitidis has demonstrated considerable microheterogeneity in the variable region of LOS due to the presence of novel glycoforms. As a step toward understanding the basis for the expression of these novel glycoforms, we have examined the LOS structures and UDP-glucose 4-epimerase (epimerase) activity levels in two strains (NMB and MA-1) and their respective galE mutants. Strain NMB was found to have low epimerase activity and to contain multiple glycoforms, some of which appear to contain only glucose sugars. The galE mutant had only the oligoglucose glycoforms. Strain MA-1 had higher ...


Circulation And Energy Metabolism In The Brain / Donald D. Clarke And Louis Sokoloff, Donald Dudley Clarke Phd, Louis Sokoloff Jan 1999

Circulation And Energy Metabolism In The Brain / Donald D. Clarke And Louis Sokoloff, Donald Dudley Clarke Phd, Louis Sokoloff

Chemistry Faculty Publications

No abstract provided.


Preparation And Fungitoxicity Of Some Dichloro-8-Quinolinols / Herman Gershon, Donald D. Clarke, And Muriel Gershon Department Of Chemistry, Fordham University, Bronx, Ny 10458, Usa New York Botanical Garden, Bronx, Ny 10458, Usa, Herman Gershon, Donald Dudley Clarke Phd, Muriel Gershon Jan 1999

Preparation And Fungitoxicity Of Some Dichloro-8-Quinolinols / Herman Gershon, Donald D. Clarke, And Muriel Gershon Department Of Chemistry, Fordham University, Bronx, Ny 10458, Usa New York Botanical Garden, Bronx, Ny 10458, Usa, Herman Gershon, Donald Dudley Clarke Phd, Muriel Gershon

Chemistry Faculty Publications

2,5-, 3,5-, 3,7-, 4,5-, 5,6-, und 6,7-Dichloro-8-quinolinols were prepared and tested along with their 3,6- and 5,7-ana1ogues against six fungi (Aspergillus niger, A. oryzae, Myrothecium verrucaria, Trichoderma viride, Mucor cirinelloides, and Trichophyton mentagrophytes) in Sabouraud dextrose broth. Most of the compounds were strongly antifungal, inhibiting five of the fungi below 1 μg/ml. This activity is attributed to intramolecular synergism. M. cirinelloides was inhibited less by these compounds


Two-Photon Excitation Of Rhenium Metal-Ligand Complexes, Joseph R. Lakowicz, Felix N. Castellano, Ignacy Gryczynski, Zygmunt Gryczynski, Jonathan D. Dattelbaum Jan 1999

Two-Photon Excitation Of Rhenium Metal-Ligand Complexes, Joseph R. Lakowicz, Felix N. Castellano, Ignacy Gryczynski, Zygmunt Gryczynski, Jonathan D. Dattelbaum

Chemistry Faculty Publications

We describe the emission spectral properties of two rhenium metal-ligand complexes with one and two-photon excitation, Re(bpy)2(CO)3Cl and [Re(bpy)(CO)3CH3CN]+, where bpy is 2,2’-bipyridyl and CH3CN is acetonitrile. Similar emission spectra and intensity decay times characteristic of the metal-to-ligand charge transfer state were observed for one- and two-photon excitation. The lifetime and quantum yield of the acetonitrile complex are approximately 14-fold higher than that of the chloride complex. Both complexes display high anisotropies near 0.33 in frozen solution with one-photon excitation. Two-photon excitation results in ...


Phorbol 12-Myristate 13-Acetate Stimulates Lysophosphatidic Acid Secretion From Ovarian And Cervical Cancer Cells But Not From Breast Or Leukemia Cells, Zhongzhou Shen, Jerome Belinson, Richard E. Morton, Yan Xu Dec 1998

Phorbol 12-Myristate 13-Acetate Stimulates Lysophosphatidic Acid Secretion From Ovarian And Cervical Cancer Cells But Not From Breast Or Leukemia Cells, Zhongzhou Shen, Jerome Belinson, Richard E. Morton, Yan Xu

Chemistry Faculty Publications

Lysophosphatidic acid (LPA) is present in ascites from patients with ovarian cancer. It stimulates calcium release and growth of ovarian cancer cells bothin vitroandin vivo.Recently, we found that LPA levels were significantly elevated in plasma from patients with ovarian cancer and other gynecological cancers. In contrast, LPA levels were not elevated in patients with breast cancer and leukemias. In view of this, we investigated whether gynecological cancer cells could produce LPA. LPA was extracted from the supernatant of cells culturedin vitroand purified by thin layer chromatography. After hydrolysis and transmethylation, the fatty acid derivatives were analyzed by gas chromatography ...


Simultaneous Formation Of Functional Leading And Lagging Strand Holoenzyme Complexes On A Small, Defined Dna Substrate, Anthony J. Berdis, Stephen J. Benkovic Sep 1998

Simultaneous Formation Of Functional Leading And Lagging Strand Holoenzyme Complexes On A Small, Defined Dna Substrate, Anthony J. Berdis, Stephen J. Benkovic

Chemistry Faculty Publications

The biochemical characterization of leading and lagging strand DNA synthesis by bacteriophage T4 replication proteins has been addressed utilizing a small, defined primer/template. The ATP hydrolysis activity of 44/62, the clamp loading complex responsible for holoenzyme assembly, was monitored during assembly of both the leading and lagging strand holoenzyme complex. The ATPase activity of 44/62 diminishes once a functional holoenzyme is assembled on both the leading and lagging strand. The assembly of the lagging strand holoenzyme is facilitated by several factors including biotinylated streptavidin blocks at the end of the fork strands, preassembly of the leading strand ...


Crystal Structures Of Copper(Ii) Complexes Of Some 2-Methyl-8-Quinolinols And Implications For Their Antifungal Activity / Massud Shoja, Herman Gershon, Diana Bray, And Donald D. Clarke Department Of Chemistry, Fordham University, Bronx, Ny 10458, Usa New York Botanical Garden, Bronx, Ny 10458, Usa, Massud Shoja Phd, Herman Gershon, Diana Bray, Donald Dudley Clarke Phd Jan 1998

Crystal Structures Of Copper(Ii) Complexes Of Some 2-Methyl-8-Quinolinols And Implications For Their Antifungal Activity / Massud Shoja, Herman Gershon, Diana Bray, And Donald D. Clarke Department Of Chemistry, Fordham University, Bronx, Ny 10458, Usa New York Botanical Garden, Bronx, Ny 10458, Usa, Massud Shoja Phd, Herman Gershon, Diana Bray, Donald Dudley Clarke Phd

Chemistry Faculty Publications

A hypothesis that the geometry of a potential fungicide must be consistent with that of the pores of the fungal spore wall in order to penetrate it and be toxic has been developed. Certain bis(8-quinolinolato)copper(II) complexes seemed to contradict this. To resolve this issue, structures of bis(7-fluoro-8-quinolinolato )copper(II) (1), bis(2-methyl-8-quinolinolato )copper(II) (2), and bis(2-methyl-7-nitro-8-quinolinolato)copper(II) (3) were solved. The ligands of 1 are square planar with copper at the center of symmetry. In 2 and 3 the methyl group at C2 interacts with the other 8- quinolinol ligand, producing a significant ...


Revision Of The Assigned Structures Of 5- And 7-Iodo-8-Quinolinols And 5- And 7-Iodo-2-Methyl-8-Quinolinols / Donald D. Clarke, Herman Gershon, Massud Shoja, And Mei-Wen Yen Department Of Chemistry, Fordham University, Bronx, Ny 10458, Usa, Donald Dudley Clarke Phd, Herman Gershon, Massud Shoja Phd, Mei-Wen Yen Jan 1998

Revision Of The Assigned Structures Of 5- And 7-Iodo-8-Quinolinols And 5- And 7-Iodo-2-Methyl-8-Quinolinols / Donald D. Clarke, Herman Gershon, Massud Shoja, And Mei-Wen Yen Department Of Chemistry, Fordham University, Bronx, Ny 10458, Usa, Donald Dudley Clarke Phd, Herman Gershon, Massud Shoja Phd, Mei-Wen Yen

Chemistry Faculty Publications

Revised structures are presented for 5- and 7-iodo-8-quinolinols and for 5- and 7-iodo-2-methyl-8-quinolinols based on NMR studies. UV spectroscopic characterization of the compounds was also carried out


Characterization Of A Transposon Tn916-Generated Mutant Of Haemophilus Ducreyi 35000 Defective In Lipooligosaccharide Biosynthesis, B W. Gibson, A A. Campagnari, William Melaugh, M A. Apicella, S Grass, Jing Wang, Katherine L. Palmer, R S. Munson Jan 1997

Characterization Of A Transposon Tn916-Generated Mutant Of Haemophilus Ducreyi 35000 Defective In Lipooligosaccharide Biosynthesis, B W. Gibson, A A. Campagnari, William Melaugh, M A. Apicella, S Grass, Jing Wang, Katherine L. Palmer, R S. Munson

Chemistry Faculty Publications

To define the role of the surface lipooligosaccharide (LOS) of Haemophilus ducreyi in the pathogenesis of chancroid, Tn916 mutants of H. ducreyi 35000 defective in expression of the murine monoclonal antibody (MAb) 3F11 epitope on H. ducreyi LOS were identified by immunologic screening. One mutant, designated 1381, has an LOS which lacks the MAb 3F11 epitope and migrates with an increased mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The gene disrupted by the Tn916 element in strain 1381 was identified by cloning the sequences flanking the Tn916 element. The sequences were then used to probe a lambda DASHII genomic library ...


Identification Of The Adp-L-Glycero-D-Manno-Heptose-6-Epimerase (Rfad) And Heptosyltransferase Ii (Rfaf) Biosynthesis Genes From Nontypeable Haemophilus Influenzae 2019, W A. Nichols, B W. Gibson, William Melaugh, N G. Lee, M Sunshine, M A. Apicella Jan 1997

Identification Of The Adp-L-Glycero-D-Manno-Heptose-6-Epimerase (Rfad) And Heptosyltransferase Ii (Rfaf) Biosynthesis Genes From Nontypeable Haemophilus Influenzae 2019, W A. Nichols, B W. Gibson, William Melaugh, N G. Lee, M Sunshine, M A. Apicella

Chemistry Faculty Publications

Haemophilus influenzae is an important human pathogen. The lipooligosaccharide (LOS) of H. influenzae has been implicated as a virulence determinant. To better understand the assembly of LOS in nontypeable H. influenzae (NtHi), we have cloned and characterized the rfaD and rfaF genes of NtHi 2019, which encode the ADP-L-glycero-D-manno-heptose-6-epimerase and heptosyltransferase II enzymes, respectively. This cloning was accomplished by the complementation of Salmonella typhimurium lipopolysaccharide (LPS) biosynthesis gene mutants. These deep rough mutants are novobiocin susceptible until complemented with the appropriate gene. In this manner, we are able to use novobiocin resistance to select for specific NtHi LOS inner core ...


Nerve Tissue-Specific Human Glutamate Dehydrogenase That Is Thermolabile And Highly Regulated By Adp / P. Shashidharan, Donald D. Clarke, Naveed Ahmed, Nicholas Moschonas, And Andreas Plaitakis Department Of Neurology, Mount Sinai School Of Medicine, New York; Department Of Chemistry, Fordham University, Bronx New York, Usa; And Department Of Biology And School Of Health Sciences, University Of Crete, Crete, Greece, P. Shashidharan, Donald Dudley Clarke Phd, Naveed Ahmed, Nicholas Moschonas Jan 1997

Nerve Tissue-Specific Human Glutamate Dehydrogenase That Is Thermolabile And Highly Regulated By Adp / P. Shashidharan, Donald D. Clarke, Naveed Ahmed, Nicholas Moschonas, And Andreas Plaitakis Department Of Neurology, Mount Sinai School Of Medicine, New York; Department Of Chemistry, Fordham University, Bronx New York, Usa; And Department Of Biology And School Of Health Sciences, University Of Crete, Crete, Greece, P. Shashidharan, Donald Dudley Clarke Phd, Naveed Ahmed, Nicholas Moschonas

Chemistry Faculty Publications

Glutamate dehydrogenase (GDH), an enzyme that is central to the metabolism of glutamate, is present at high levels in the mammalian brain. Studies on human leukocytes and rat brain suggested the presence of two GDH activities differing in thermal stability and allosteric regulation, but molecular biological investigations led to the cloning of two human GDH-specific genes encoding highly homologous polypeptides. The first gene, designated GLUD1, is expressed in all tissues (housekeeping GDH), whereas the second gene, designated GLUD2, is expressed specifically in neural and testicular tissues. In this study, we obtained both GDH isoenzymes in pure form by expressing a ...


Protein-Protein And Protein-Dna Interactions At The Bacteriophage T4 Dna Replication Fork. Characterization Of A Fluorescently Labeled Dna Polymerase Sliding Clamp, Daniel J. Sexton, Theodore E. Carver, Anthony J. Berdis, Stephen J. Benkovic Nov 1996

Protein-Protein And Protein-Dna Interactions At The Bacteriophage T4 Dna Replication Fork. Characterization Of A Fluorescently Labeled Dna Polymerase Sliding Clamp, Daniel J. Sexton, Theodore E. Carver, Anthony J. Berdis, Stephen J. Benkovic

Chemistry Faculty Publications

The T4 DNA polymerase holoenzyme is composed of the polymerase enzyme complexed to the sliding clamp (the 45 protein), which is loaded onto DNA by an ATP-dependent clamp loader (the 44/62 complex). This paper describes a new method to directly investigate the mechanism of holoenzyme assembly using a fluorescently labeled cysteine mutant of the 45 protein. This protein possessed unaltered function yet produced substantial changes in probe fluorescence intensity upon interacting with other components of the holoenzyme. These fluorescence changes provide insight into the role of ATP hydrolysis in holoenzyme assembly. Using either ATP or the non-hydrolyzable ATP analog ...


The Carboxyl Terminus Of The Bacteriophage T4 Dna Polymerase Is Required For Holoenzyme Complex Formation, Anthony J. Berdis, Patrice Soumillion, Stephen J. Benkovic Nov 1996

The Carboxyl Terminus Of The Bacteriophage T4 Dna Polymerase Is Required For Holoenzyme Complex Formation, Anthony J. Berdis, Patrice Soumillion, Stephen J. Benkovic

Chemistry Faculty Publications

To further elucidate the mechanism and dynamics of bacteriophage T4 holoenzyme formation, a mutant polymerase in which the last six carboxyl-terminal amino acids are deleted, was constructed, overexpressed, and purified to homogeneity. The mutant polymerase, designated ΔC6 exo−, is identical to wild-type exo− polymerase with respect to kcat, kpol, and dissociation constants for nucleotide and DNA substrate. However, unlike wild-type exo− polymerase, the ΔC6 exo− polymerase is unable to interact with the 45 protein to form the stable holoenzyme. A synthetic polypeptide corresponding to the carboxyl terminus of the wild-type exo− polymerase was tested as an in vitro inhibitor of ...


The Lipooligosaccharides Of Haemophilus Ducreyi Are Highly Sialylated, William Melaugh, A A. Campagnari, B W. Gibson Jan 1996

The Lipooligosaccharides Of Haemophilus Ducreyi Are Highly Sialylated, William Melaugh, A A. Campagnari, B W. Gibson

Chemistry Faculty Publications

The major lipooligosaccharides of the sexually transmitted pathogen Haemophilus ducreyi 35000 have been previously found to terminate in N-acetyllactosamine and sialyl-N-acetyllactosamine, Neu5Ac alpha 2-->3Gal beta 1-->4GlcNAc (W. Melaugh, N. J. Phillips, A. A. Campagnari, M. V. Tullius, and B. W. Gibson, Biochemistry 33: 13070-13078, 1994). In this study, mass spectrometry and composition analyses have shown that the lipooligosaccharides from three other H. ducreyi strains also contain N-acetyllactosamine and are highly sialylated (approximately 30 to 50%), although one African strain was found to contain neither of these structural features.