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Biochemistry, Biophysics, and Structural Biology Commons

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Chemistry Faculty Publications

1957

Articles 1 - 3 of 3

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Metabolic Activity Of Protein Amide Groups, Donald Dudley Clarke Phd, Amos Neidle, Nirmal K. Sarkar, Heinrich Waelsch Jan 1957

Metabolic Activity Of Protein Amide Groups, Donald Dudley Clarke Phd, Amos Neidle, Nirmal K. Sarkar, Heinrich Waelsch

Chemistry Faculty Publications

No abstract provided.


An Enzymically Catalyzed Incorporation Of Amines Into Proteins / Nirmal K. Sarkar Donald D. Clarke Heinrich Waelsch Department Of Biochemistry, College Of Physicians And Surgeons, Columbia University, New York, And The New York Psychiatric Institute, New York, N.Y. (U.S.A.), Nirmal K. Sarkar, Donald Dudley Clarke Phd, Heinrich Waelsch Jan 1957

An Enzymically Catalyzed Incorporation Of Amines Into Proteins / Nirmal K. Sarkar Donald D. Clarke Heinrich Waelsch Department Of Biochemistry, College Of Physicians And Surgeons, Columbia University, New York, And The New York Psychiatric Institute, New York, N.Y. (U.S.A.), Nirmal K. Sarkar, Donald Dudley Clarke Phd, Heinrich Waelsch

Chemistry Faculty Publications

In experiments reported some years ago the enzyme-catalyzed exchange of the amide group of glutamine and asparagine with 15N ammonia was demonstrated but an exchange of the amide groups of protein bound dicarboxylic amino acids could not be explored owing to the unfavorable experimental conditions1 . Recently the problem of the metabolism of protein-amide groups was approached again with the aid of 14C-labeled amines, such as ethanolamine or cadaverine. Soluble protein fractions obtained from sucrose homogenates (o.2sM) of the livers of guinea pigs, rats, mice, and rabbits by centrifugation at roo,ooo X g were incubated in the presence of ...


Synthesis Of Phosphatidyl Peptides I. O-(Distearoyl-L-A-Glycerylphosphoryl)-L-Serylglycylglycine / By Erich Baer, Jonas Maurukas, And Donald D. Clarke (From The Banting And Best Department Of Medical Research, University Of Toronto, Toronto, Canada), Erich Baer, Jonas Maurukas, Donald Dudley Clarke Phd Jan 1957

Synthesis Of Phosphatidyl Peptides I. O-(Distearoyl-L-A-Glycerylphosphoryl)-L-Serylglycylglycine / By Erich Baer, Jonas Maurukas, And Donald D. Clarke (From The Banting And Best Department Of Medical Research, University Of Toronto, Toronto, Canada), Erich Baer, Jonas Maurukas, Donald Dudley Clarke Phd

Chemistry Faculty Publications

The synthesis of a phosphatidyl tripeptide has been accomplished. It was prepared by (a) phosphorylation of D-α,β-distearin with phenylphosphoryl dichloride and pyridine, (b) esterification of the resulting distearoylL- α-glycerylphenylphosphoryl chloride with N-carbobenzoxy-L-serylglycylglycine benzyl ester in the presence of lutidine, and (c) removal of the protective groups by catalytic hydrogenolysis. The O-( distearoyl-L-α-glycerylphosphoryl)- L-serylglycylglycine was obtained in an over-all yield of 12 per cent. The synthesis of N-carbobenzoxy-L-serylglycylglycine benzyl ester, for which two procedures have been reported, has been simplified considerably by condensing N -carbobenzoxy-L-serine with glycylglycine benzyl ester by means of N ,N' -dicyclohexylcarbodiimide. The phosphatidyl tripeptide is cleaved ...