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Biochemistry, Biophysics, and Structural Biology Commons

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Chemistry Faculty Publications

Fordham University

Glutamate

1967

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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Labeling Of Glutamate, Glutamine, And Aspartate In Brain From C14 Aspartate And Acetate / W. J. Ncklas D. D. Clarke And S. Berl Chemistry Dept., Fordham University And Col. Of Physicians And Surgeons, Columbia University, William J. Nicklas, Donald Dudley Clarke Phd, Soll Berl Jan 1967

Labeling Of Glutamate, Glutamine, And Aspartate In Brain From C14 Aspartate And Acetate / W. J. Ncklas D. D. Clarke And S. Berl Chemistry Dept., Fordham University And Col. Of Physicians And Surgeons, Columbia University, William J. Nicklas, Donald Dudley Clarke Phd, Soll Berl

Chemistry Faculty Publications

Guinea pig brain cortex slices incubated in media containing U-C14 aspartic acid form glutamine of higher specific activity than glutamic acid (Fed. Proc. -25, 713 (1966) ). Glutamic acid and glutamine from such experiments were enzymatically decarboxylated and the o/o of label in C-1 ranged from 37 to 42o/o. These values exceed the maximum predicted from the known operation of the citric acid cycle viz. 33o/o. When l-C14 acetate was injected into mice or incubated with brain slices the aspartate isolated from brain had more than 50o/o of the label in C-4. From the known operation of ...