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Biochemistry, Biophysics, and Structural Biology Commons

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Chemistry Faculty Publications

Fordham University

1986

Articles 1 - 2 of 2

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Purification And Characterization Of A Soluble And A Particulate Glutamate Dehydrogenase From Rat Brain / Arlene D. Colon, Andreas Plaitakis, Antonis Perakis, Soll Berl, And Donald D. Clarke Department Of Neurology, Mount Sinai School Of Medicine, New York, And Department Of Chemistry, Fordham University, Bronx, New York, U.S.A., Arlene D. Colon, Andreas Plaitakis, Antonis Perakis, Donald Dudley Clarke Phd Jan 1986

Purification And Characterization Of A Soluble And A Particulate Glutamate Dehydrogenase From Rat Brain / Arlene D. Colon, Andreas Plaitakis, Antonis Perakis, Soll Berl, And Donald D. Clarke Department Of Neurology, Mount Sinai School Of Medicine, New York, And Department Of Chemistry, Fordham University, Bronx, New York, U.S.A., Arlene D. Colon, Andreas Plaitakis, Antonis Perakis, Donald Dudley Clarke Phd

Chemistry Faculty Publications

Glutamate dehydrogenase (GDH) activity was determined in high-speed fractions (1 00,000 g for 60 min) obtained from whole rat brain homogenates after removal of a low-speed pellet (480 g for 10 min). Approximately 60% of the high-speed GDH activity was particulate (associated with membrane) and the remaining was soluble (probably of mitochondrial matrix origin). Most of the particulate GDH activity resisted extraction by several commonly used detergents, high concentration of salt, and sonication; however, it was largely extractable with the cationic detergent cetyltrimethylammonium bromide (CTAB) in hypotonic buffer solution. The two GDH activities were purified using a combination of ...


The Subcellular Localization Of Glutamate Dehydrogenase (Gdh): Is Gdh A Marker For Mitochondria In Brain? / James C. K. Lai, Kwan-Fu Rex Sheu, Young Tai Kim, Donald D. Clarke, And John P. Blass Department Of Neurology, Cornell University Medical College And Altschul Laboratory For Dementia Research Burke Rehabilitation Center White Plains, Ny 10605 And Department Of Medicine Cornell University Medical College New York, Ny 10021, James C. K. Lai, Kwan-Fu Rex Sheu, Young Tai Kim, Donald Dudley Clarke Phd Jan 1986

The Subcellular Localization Of Glutamate Dehydrogenase (Gdh): Is Gdh A Marker For Mitochondria In Brain? / James C. K. Lai, Kwan-Fu Rex Sheu, Young Tai Kim, Donald D. Clarke, And John P. Blass Department Of Neurology, Cornell University Medical College And Altschul Laboratory For Dementia Research Burke Rehabilitation Center White Plains, Ny 10605 And Department Of Medicine Cornell University Medical College New York, Ny 10021, James C. K. Lai, Kwan-Fu Rex Sheu, Young Tai Kim, Donald Dudley Clarke Phd

Chemistry Faculty Publications

Glutamate dehydrogenase (GDH, EC 1.4.1.2) has long been used as a marker for mitochondria in brain and other tissues, despite reports indicating that GDH is also present in nuclei of liver and dorsal root ganglia. To examine whether GDH can be used as a marker to differentiate between mitochondria and nuclei in the brain, we have measured GDH by enzymatic activity and on immunoblots in rat brain mitochondria and nuclei which were highly enriched by density-gradient centrifugation methods. The activity of GDH was enriched in the nuclear fraction as well as in the mitochondrial fraction, while the ...