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Biochemistry, Biophysics, and Structural Biology Commons

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Chemistry Faculty Publications

Biochemistry

1967

Articles 1 - 2 of 2

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Labeling Of Glutamate, Glutamine, And Aspartate In Brain From C14 Aspartate And Acetate / W. J. Ncklas D. D. Clarke And S. Berl Chemistry Dept., Fordham University And Col. Of Physicians And Surgeons, Columbia University, William J. Nicklas, Donald Dudley Clarke Phd, Soll Berl Jan 1967

Labeling Of Glutamate, Glutamine, And Aspartate In Brain From C14 Aspartate And Acetate / W. J. Ncklas D. D. Clarke And S. Berl Chemistry Dept., Fordham University And Col. Of Physicians And Surgeons, Columbia University, William J. Nicklas, Donald Dudley Clarke Phd, Soll Berl

Chemistry Faculty Publications

Guinea pig brain cortex slices incubated in media containing U-C14 aspartic acid form glutamine of higher specific activity than glutamic acid (Fed. Proc. -25, 713 (1966) ). Glutamic acid and glutamine from such experiments were enzymatically decarboxylated and the o/o of label in C-1 ranged from 37 to 42o/o. These values exceed the maximum predicted from the known operation of the citric acid cycle viz. 33o/o. When l-C14 acetate was injected into mice or incubated with brain slices the aspartate isolated from brain had more than 50o/o of the label in C-4. From the known operation of ...


Determination Of Urinary 3-Methoxy-4-Hydroxyphenylethylene Glycol By Gas-Liquid Chromatography And Electron Capture Detection / Sherwin Wilk, Stanley E. Gitlow, Donald D. Clarke And David H. Paley Department Of Medicine, The Mount Sinai Hospital, New York, And Department Of Chemistry, Fordham University, New York, N.Y. (U.S.A.), Sherwin Wilk, Stanley E. Gitlow, Donald Dudley Clarke Phd, David H. Paley Jan 1967

Determination Of Urinary 3-Methoxy-4-Hydroxyphenylethylene Glycol By Gas-Liquid Chromatography And Electron Capture Detection / Sherwin Wilk, Stanley E. Gitlow, Donald D. Clarke And David H. Paley Department Of Medicine, The Mount Sinai Hospital, New York, And Department Of Chemistry, Fordham University, New York, N.Y. (U.S.A.), Sherwin Wilk, Stanley E. Gitlow, Donald Dudley Clarke Phd, David H. Paley

Chemistry Faculty Publications

The determination of urinary 3-methoxy-4-hydroxyphenylethylene glycol (HMPG), a major metabolite of epinephrine and norepinephrine by gas-liquid chromatography and electron capture detection is described. A purified extract of HMPG was treated with trifluoroacetic anhydride to form the highly sensitive HMPG-trifluoroacetate derivative. Chromatography was performed on a 6% QF-I- 0065 column at 158°. Recovery was found to be 55·4% using [3H]HMPG. Two sets of eight identical samples demonstrated a standard deviation from the mean of 6.9% and 5.7%. Thirty-five normal human urine specimens assayed by this procedure gave a mean HMPG excretion of 0.86 #gfmg creatinine (range ...