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Articles 1 - 10 of 10

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

The Effects Of Cadmium On Breast Cancer Progression, Maggie Louie Oct 2010

The Effects Of Cadmium On Breast Cancer Progression, Maggie Louie

Maggie Louie

No abstract available


Erythrocyte Aggregation And Neutrophil Function In An Aging Population, Rhys Christy, Oguz Baskurt, Gregory Gass, A. Gray, Sonya Marshall-Gradisnik Sep 2010

Erythrocyte Aggregation And Neutrophil Function In An Aging Population, Rhys Christy, Oguz Baskurt, Gregory Gass, A. Gray, Sonya Marshall-Gradisnik

A. Bon Gray

There are limited investigations which have examined the relationship between neutrophil activation and erythrocyte aggregation in older persons. The purpose of the present study was to investigate the relationship between neutrophil activation and erythrocyte aggregation (EA) in an aging population. Twenty-eight male and female subjects were allocated into one of four groups with 7 participants in each group (group 1, 20–29 years; group 2, 30–39 years; group 3, 40–49 years; group 4, 50–59 years). EA was determined using the Myrenne aggregometer. Neutrophil function (respiratory burst and phagocytic activity) was assessed using flow cytometry. EA was found ...


Autonomous Bioluminescent Expression Of The Bacterial Luciferase Gene Cassette (Lux) In A Mammalian Cell Line, Dan Close, Patterson, Ripp, Seung Baek, Sanseverino, Gary Sayler Jul 2010

Autonomous Bioluminescent Expression Of The Bacterial Luciferase Gene Cassette (Lux) In A Mammalian Cell Line, Dan Close, Patterson, Ripp, Seung Baek, Sanseverino, Gary Sayler

Seung J Baek

No abstract provided.


Erythrocyte Aggregation And Neutrophil Function In An Aging Population, Rhys Christy, Oguz Baskurt, Gregory Gass, A. Gray, Sonya Marshall-Gradisnik Jul 2010

Erythrocyte Aggregation And Neutrophil Function In An Aging Population, Rhys Christy, Oguz Baskurt, Gregory Gass, A. Gray, Sonya Marshall-Gradisnik

Sonya Marshall-Gradisnik

There are limited investigations which have examined the relationship between neutrophil activation and erythrocyte aggregation in older persons. The purpose of the present study was to investigate the relationship between neutrophil activation and erythrocyte aggregation (EA) in an aging population. Twenty-eight male and female subjects were allocated into one of four groups with 7 participants in each group (group 1, 20–29 years; group 2, 30–39 years; group 3, 40–49 years; group 4, 50–59 years). EA was determined using the Myrenne aggregometer. Neutrophil function (respiratory burst and phagocytic activity) was assessed using flow cytometry. EA was found ...


Large Scale Reverse Genetics In Arabidopsis: Case Studies From The Chloroplast 2010 Project, Imad Ajjawi, Yan Lu, Linda Savage, Shannon Bell, Robert Last Jan 2010

Large Scale Reverse Genetics In Arabidopsis: Case Studies From The Chloroplast 2010 Project, Imad Ajjawi, Yan Lu, Linda Savage, Shannon Bell, Robert Last

Yan Lu

No abstract provided.


Cell Adhesion Property Affected By Cyclooxygenase And Lipoxygenase: Opto-Electric Approach (With Chang Kyoung Choi, Mugdha Sukhthankar, Chul-Ho Kim, Seong-Ho Lee, Anthony English, Kenneth D. Kihm., Seung Baek Dec 2009

Cell Adhesion Property Affected By Cyclooxygenase And Lipoxygenase: Opto-Electric Approach (With Chang Kyoung Choi, Mugdha Sukhthankar, Chul-Ho Kim, Seong-Ho Lee, Anthony English, Kenneth D. Kihm., Seung Baek

Seung J Baek

Expression of cyclooxygenases (COX) and lipoxygenases (LOX) has been linked to many pathophysiological phenotypes, including cell adhesion. However, many current approaches to measure cellular changes are performed only in a fixed-time point. Since cells dynamically move in conjunction with the cell matrix, there is a pressing need for dynamic or time-dependent methods for the investigation of cell properties. In the presented study, we used stable human colorectal cancer cell lines ectopically expressing COX-1, COX-2, and 15LOX-1, to investigate whether expression of COX-1, COX-2, or 15LOX-1 would affect cell adhesion using our opto-electric methodology. In a fixed-time point experiment, only COX-1- ...


A Potential Proliferative Gene, Nudt6, Is Down-Regulated By Green Tea Catechins At The Posttranscriptional Level, Seung Baek, Mugdha Sukthankar, Ck Choi, A English, Js Kim Dec 2009

A Potential Proliferative Gene, Nudt6, Is Down-Regulated By Green Tea Catechins At The Posttranscriptional Level, Seung Baek, Mugdha Sukthankar, Ck Choi, A English, Js Kim

Seung J Baek

The main aims of this study were to elucidate the effect of green tea catechins on Nudix-type motif 6 (NUDT6) suppression and to characterize NUDT6's biological activity. Our microarray data showed that the green tea component epicatechin-3-gallate suppressed NUDT6 expression, and this was confirmed by RT-PCR. Subsequently, the use of different catechins showed that the effect of epigallocatechin-3-gallate (EGCG) was stronger than that of other catechins. At the posttranscriptional level, EGCG decreased the RNA stability of NUDT6, indicating it as a potential mechanism of NUDT6 suppression. Further cloning of the 3' untranslated region of human NUDT6 mRNA resulted in ...


A Bird’S-Eye View On The Function Of Sleep, Charles Amlaner, Niels Rattenborg Dec 2009

A Bird’S-Eye View On The Function Of Sleep, Charles Amlaner, Niels Rattenborg

Charles J. Amlaner

Sleep has been detected in every animal that has been adequately studied (Cirelli & Tononi, 2008). The ubiquitous nature of sleep suggests that it evolved early in the course of evolution and therefore may serve a conserved function essential to all animals. This hypothesis forms the rationale behind the development of “simple” animal models of sleep (Allada & Siegel, 2008; Mignot, 2008). By studying sleep in animals such as the fruit fly (Drosophila melanogaster), where the power of genetic techniques can be readily employed,we may gain insight into the initial (perhaps cellular) function of sleep, a function that may still be relevant to understanding sleep in humans. Indeed, recent studies have already demonstrated remarkable similarities between sleep in Drosophila and sleep in mammals (Hendricks, Finn, Panckeri, et al., 2000; Shaw, Cirelli, Greenspan, et al., 2000; reviewed in Cirelli & Bushey, 2008). Although the utility of studying sleep in“simple”animal models is undeniable, it is unlikely that this approach alone will tell the whole story, especially given that Drosophilado not exhibit brain states comparable to mammalian slow-wave sleep (SWS) and rapid eye-movement (REM) sleep (Cirelli, 2006; Cirelli & Bushey, 2008; Hendricks & Sehgal, 2004; Nitz, van Swinderen, Tononi, et al ...


Solution-Phase Submonomer Diversification Of Aza-Dipeptide Building Blocks And Their Application In Aza-Peptide And Aza-Diketopiperazine Synthesis, Carine Bourguet, Caroline Proulx, Sophie Klocek, David Sabatino, William Lubell Dec 2009

Solution-Phase Submonomer Diversification Of Aza-Dipeptide Building Blocks And Their Application In Aza-Peptide And Aza-Diketopiperazine Synthesis, Carine Bourguet, Caroline Proulx, Sophie Klocek, David Sabatino, William Lubell

David Sabatino

No abstract provided.


In Vitro Anti-Proliferative Activity Of Alcoholic Stem Extract Of Coscinium Fenestratum In Human Colorectal Cancer Cells, P Rojsanga, M Sukthankar, C Krisanapun, W Gritsanapan, D Lawson, Seung Baek Dec 2009

In Vitro Anti-Proliferative Activity Of Alcoholic Stem Extract Of Coscinium Fenestratum In Human Colorectal Cancer Cells, P Rojsanga, M Sukthankar, C Krisanapun, W Gritsanapan, D Lawson, Seung Baek

Seung J Baek

Coscinium fenestratum (Gaertn.) Colebr. is traditionally used for the treatment of cancer, arthritis and diabetes mellitus. The purpose of this study was to determine the molecular mechanisms by which this plant shows beneficial effects. An 80% ethanolic extract of C. fenestratum (80ET) was separated by its polarity into dichloromethane (DCM) and aqueous fractions (WF), and the anti-proliferative effects of 80ET, DCM and WF were investigated. Berberine, one of the major components of C. fenestratum, was used as a control. The 80ET, DCM, WF and berberine showed anti-proliferative activity as assessed by cell growth assay. Subsequently, the pro-apoptotic proteins NAG-1 and ...