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Biochemistry, Biophysics, and Structural Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

None

2009

Nutrition

Activating Transcription Factor 3

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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

The Cyclooxygenase Inhibitor Sulindac Sulfide Inhibits Ep4 Expression And Suppresses The Growth Of Glioblastoma Cells, A Kambe, H Yoshioka, H Kamitani, T Watanabe, Seung Baek, T Eling Dec 2008

The Cyclooxygenase Inhibitor Sulindac Sulfide Inhibits Ep4 Expression And Suppresses The Growth Of Glioblastoma Cells, A Kambe, H Yoshioka, H Kamitani, T Watanabe, Seung Baek, T Eling

Seung J Baek

EP4 expression in human glioblastoma cells correlates with growth on soft agar. The cyclooxygenase inhibitor sulindac sulfide first altered specificity protein-1 (Sp-1) and early growth response gene-1 expression, then increased the expression of nonsteroidal anti-inflammatory drug-activated gene 1 and activating transcription factor 3, and then decreased EP4 expression. EP4 suppression was dependent on blocking the Sp-1 binding sites in the human EP4 promoter. Mutation in the Sp-1 sites in EP4 altered the promoter activity and abolished sulindac sulfide effects. The inhibitory effect of sulindac sulfide on EP4 expression was reversed by PD98059, a mitogen-activated protein/extracellular signal-regulated kinase kinase-1/extracellular ...