Open Access. Powered by Scholars. Published by Universities.®

Biochemistry, Biophysics, and Structural Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 9 of 9

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Acute And Chronic Cadmium Exposure Promotes E-Cadherin Degradation In Mcf7 Breast Cancer Cells, Esmeralda Ponce, Maggie Louie, Mary B. Sevigny Sep 2015

Acute And Chronic Cadmium Exposure Promotes E-Cadherin Degradation In Mcf7 Breast Cancer Cells, Esmeralda Ponce, Maggie Louie, Mary B. Sevigny

Maggie Louie

Cadmium is an environmental carcinogen that usually enters the body at minute concentrations through diet or cigarette smoke and bioaccumulates in soft tissues. In past studies, cadmium has been shown to contribute to the development of more aggressive cancer phenotypes including increased cell migration and invasion. This study aims to determine if cadmium exposure-both acute and chronic-contributes to breast cancer progression by interfering with the normal functional relationship between E-cadherin and β-catenin. An MCF7 breast cancer cell line (MCF7-Cd) chronically exposed to 10(-7)  M CdCl2 was previously developed and used as a model system to study chronic exposures, whereas ...


Synthesis And Evaluation Of The Diarylthiourea Analogs As Novel Anti-Cancer Agents, Shengquan Liu, Maggie C. Louie, Vanishree Rajagopalan, Guangyan Zhou, Esmeralda Ponce, Tran Nguyen, Linda Green Mar 2015

Synthesis And Evaluation Of The Diarylthiourea Analogs As Novel Anti-Cancer Agents, Shengquan Liu, Maggie C. Louie, Vanishree Rajagopalan, Guangyan Zhou, Esmeralda Ponce, Tran Nguyen, Linda Green

Maggie Louie

en p-nitrodiarylthiourea analogs were designed, synthesized and evaluated in breast (MCF-7, T-47D, MDA-MB-453) and prostate (DU-145, PC-3, LNCaP) cancer cell lines for their anticancer activities. The majority of the compounds were able to inhibit the growth of these six cancer cell lines at low micromolar concentrations. Compound 7 was found to be the most potent anticancer agent in this series with GI50 values of 3.16 μM for MCF-7, 2.53 μM for T-47D, 4.77 μM for MDA-MB-453 breast cancer lines and 3.54 μM for LNCaP prostate cancer cell line. These GI50 values were comparable to the parent ...


Alternative Career Paths, Maggie Louie Nov 2014

Alternative Career Paths, Maggie Louie

Maggie Louie

No abstract available


Chronic Cadmium Exposure Promotes Breast Cancer Progression, Maggie Louie Sep 2012

Chronic Cadmium Exposure Promotes Breast Cancer Progression, Maggie Louie

Maggie Louie

No abstract available


Glycosylation Of Human Cyclooxygenase-2 (Cox-2) Decreases The Efficacy Of Certain Cox-2 Inhibitors, Mary Sevigny, Kamara Graham, Esmeralda Ponce, Maggie Louie, Kylie Mitchell Mar 2012

Glycosylation Of Human Cyclooxygenase-2 (Cox-2) Decreases The Efficacy Of Certain Cox-2 Inhibitors, Mary Sevigny, Kamara Graham, Esmeralda Ponce, Maggie Louie, Kylie Mitchell

Maggie Louie

Prostanoids play an important role in a variety of physiological and pathophysiological processes including inflammation and cancer. The rate-limiting step in the prostanoid biosynthesis pathway is catalyzed by cyclooxygenase-2 (COX-2). COX-2 exists as two glycoforms, 72 and 74 kDa, the latter resulting from an additional glycosylation at Asn580. In this study, Asn580 was mutated, and the mutant and wild-type COX-2 genes were expressed in COS-1 cells to determine how glycosylation affects the inhibition of COX-2 activity by aspirin, flurbiprofen, ibuprofen, celecoxib, and etoricoxib. Results indicate that certain inhibitors were 2–5 times more effective at inhibiting COX-2 activity when the ...


Cadmium As An Estrogen Receptor Modulator, Maggie Louie May 2011

Cadmium As An Estrogen Receptor Modulator, Maggie Louie

Maggie Louie

No abstract available


The Effects Of Cadmium On Breast Cancer Progression, Maggie Louie Oct 2010

The Effects Of Cadmium On Breast Cancer Progression, Maggie Louie

Maggie Louie

No abstract available


Proto-Oncogene Actr/Aib1 Promotes Cancer Cell Invasion By Up-Regulating Specific Matrix Metalloproteinase Expression, Li B. Li, Maggie C. Louie, Hong-Wu Chen, June X. Zou Mar 2008

Proto-Oncogene Actr/Aib1 Promotes Cancer Cell Invasion By Up-Regulating Specific Matrix Metalloproteinase Expression, Li B. Li, Maggie C. Louie, Hong-Wu Chen, June X. Zou

Maggie Louie

Overexpression of ACTR/AIB1 is frequently found in different cancers with distant metastasis. To address its possible involvement in tumor metastasis, we performed invasion assays to examine the effect of ACTR alteration on the invasiveness of breast cancer cells (MDA-MB-231 or T-47D) and found that high levels of ACTR are required for their strong invasiveness. Molecular analysis indicates that ACTR functions as a coactivator of AP-1 to up-regulate the expression of matrix metalloproteinases such as MMP-7 and MMP-10 and reduce cell adhesion to specific extracellular matrix proteins. These novel findings provide a mechanistic link between ACTR and MMPs, and suggest ...


Gene Expression In Cadmium-Tolerant Datura Innoxia: Detection And Characterization Of Cdnas Induced In Response To Cd2+, Maggie Louie, Nathan Kondor, Jane G. Dewitt Apr 2003

Gene Expression In Cadmium-Tolerant Datura Innoxia: Detection And Characterization Of Cdnas Induced In Response To Cd2+, Maggie Louie, Nathan Kondor, Jane G. Dewitt

Maggie Louie

The response of a metal tolerant plant to heavy metal stress involves a number of biochemical pathways. To investigate the overall molecular response of a metal-tolerant plant to heavy-metal exposure, suppressive subtractive hybridization was used to create a library enriched in cadmium-induced cDNAs from cadmium-tolerant Datura innoxia. Two differential screening steps were used to screen the cadmium-induced library resulting in 8 putative cadmium-specific cDNAs out of a pool of 94 clones. Reverse transcriptase polymerase chain reaction was used to confirm that 4 of these 8 clones were cadmium-specific, while the other 4 were induced under heat shock or in the ...