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Articles 571 - 600 of 619

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Asynchronous Eye-Closure And Unihemispheric Quiet Sleep Of Birds, Nigel Ball, Charles Amlaner, James Shaffery, Mark Opp Dec 1987

Asynchronous Eye-Closure And Unihemispheric Quiet Sleep Of Birds, Nigel Ball, Charles Amlaner, James Shaffery, Mark Opp

Charles J. Amlaner

No abstract provided.


Sleep Ethology In Glaucous-Winged Gull Chicks (Larus Glaucescens), James Shaffery, Nigel Ball, Charles Amlaner, Mark Opp Dec 1987

Sleep Ethology In Glaucous-Winged Gull Chicks (Larus Glaucescens), James Shaffery, Nigel Ball, Charles Amlaner, Mark Opp

Charles J. Amlaner

No abstract provided.


Thermoregulation And Sleep: Effects Of Thermal Stress On Sleep Patterns Of Glaucous-Winged Gulls (Larus Glaucescens), Mark Opp, Nigel Ball, Don Miller, Charles Amlaner Jul 1987

Thermoregulation And Sleep: Effects Of Thermal Stress On Sleep Patterns Of Glaucous-Winged Gulls (Larus Glaucescens), Mark Opp, Nigel Ball, Don Miller, Charles Amlaner

Charles J. Amlaner

1. To determine effects of thermal stress on avian sleep patterns, incubating Glaucous-winged gulls were subjected to conditions of heat loss and heat gain via conduction from hollow copper eggs.

2. Heated manipulations resulted in significant reductions in sleep and rest relative to controls, whereas cooled manipulations had little effect.

3. The resilience of sleep to thermal stress is greater than that of rest. We suggest that the incompatability of sleep with conductive processes to off-load heat from the eggs is responsible in this case.

4. Pant Sleep, the behaviour in which birds appear to maintain some the benefits of ...


Current Animal Welfare Policies And Their Effect On Biotelemetry Research: An Introduction, Charles Amlaner Dec 1986

Current Animal Welfare Policies And Their Effect On Biotelemetry Research: An Introduction, Charles Amlaner

Charles J. Amlaner

No abstract provided.


A Miniature Biotelemetry Transmitter And Automated Receiving System For The Study Of Sleep In Wild Birds, Charles Amlaner, K. Carrington, R. Shaw, D. Loenneke Dec 1986

A Miniature Biotelemetry Transmitter And Automated Receiving System For The Study Of Sleep In Wild Birds, Charles Amlaner, K. Carrington, R. Shaw, D. Loenneke

Charles J. Amlaner

No abstract provided.


24-Hour Activity Patterns Of The Douglas Squirrel, D. Peshka, Charles Amlaner, David Stewart Dec 1986

24-Hour Activity Patterns Of The Douglas Squirrel, D. Peshka, Charles Amlaner, David Stewart

Charles J. Amlaner

No abstract provided.


A Simulated Quail Population, David Stewart, Charles Amlaner, Claude Barnett Dec 1986

A Simulated Quail Population, David Stewart, Charles Amlaner, Claude Barnett

Charles J. Amlaner

No abstract provided.


Wildlife Telemetry, Charles Amlaner Dec 1986

Wildlife Telemetry, Charles Amlaner

Charles J. Amlaner

No abstract provided.


Quantitation And Identification Of Organic N-Chloramines Formed In Stomach Fluid On Ingestion Of Aqueous Hypochlorite, Frank E. Scully Jr., Katherine Mazina, Daniel E. Sonenshine, Frederick Kopfler Jan 1986

Quantitation And Identification Of Organic N-Chloramines Formed In Stomach Fluid On Ingestion Of Aqueous Hypochlorite, Frank E. Scully Jr., Katherine Mazina, Daniel E. Sonenshine, Frederick Kopfler

Biological Sciences Faculty Publications

The chemical reactions that hypochlorite undergoes in the body when chlorinated water is ingested have received very little attention. Because amino nitrogen compounds are important components of the average diet, the reactions of hypochlorite with amino compounds in the stomach were investigated. Stomach fluid was recovered from Sprague-Dawley rats that had been fasted for 48 hr and administered 4 mL deionized water. The chlorine demand of the stomach fluid was determined. An average volume-independent demand of 2.7 mg chlorine was measured. At doses below 40 mg/L chlorine reducing reactions appeared to account for reduction of all oxidizing species ...


Sleep In Birds, Charles Amlaner Dec 1984

Sleep In Birds, Charles Amlaner

Charles J. Amlaner

No abstract provided.


Radio Tracking And Telemetry, Charles Amlaner Dec 1984

Radio Tracking And Telemetry, Charles Amlaner

Charles J. Amlaner

No abstract provided.


Sleeping Gulls And Predator Avoidance, Nigel Ball, James Shaffer, Charles Amlaner Oct 1984

Sleeping Gulls And Predator Avoidance, Nigel Ball, James Shaffer, Charles Amlaner

Charles J. Amlaner

No abstract provided.


Double-Stranded Ribonucleic Acid Killer Systems In Yeasts, Donald J. Tipper, Kieth A. Bostian Jun 1984

Double-Stranded Ribonucleic Acid Killer Systems In Yeasts, Donald J. Tipper, Kieth A. Bostian

Microbiology and Physiological Systems Publications and Presentations

Killer yeasts secrete polypeptide toxins which kill sensitive cells of their own species and frequently those of other species and genera of yeast. In this review we will focus on the molecular biology of the most intensively investigated killer system, the M1- dsRNA-determined Kl killer system of S. cerevisiae. We hope to demonstrate the relevance of this system to many of the vital issues in yeast and general eucaryotic molecular biology.


Taking Laboratory Experiments Into The Field: Studies Of Sleep In Wild Animals, Charles Amlaner Dec 1983

Taking Laboratory Experiments Into The Field: Studies Of Sleep In Wild Animals, Charles Amlaner

Charles J. Amlaner

No abstract provided.


Effect Of Carbon Dioxide On The Molting Activity Of Two Tick Species In The Laboratory, Kenneth H. Holscher, R. W. Barker Sep 1983

Effect Of Carbon Dioxide On The Molting Activity Of Two Tick Species In The Laboratory, Kenneth H. Holscher, R. W. Barker

Entomology Publications

The effects of carbon dioxide concentration on the molting activity of Amblyomma americana (L.), and Amblyomma maculatum Koch were investigated. In both species, replete nymphs held in humidity chambers at a high carbon dioxide concentration (ca. 900 pp,) had a 2-4 day delay in peak ecdysis when compared to nymphs held at a low carbon dioxide concentration (ca. 400 ppm). In contrast, replete larvae showed no apparent delay in peak ecydsis [sic.] as a result of increased carbon dioxide concentration. Percent total eclosion was not affected by carbon dioxide concentration in either nymphs or larvae.


Secretion Of Saccharomyces Cerevisiae Killer Toxin: Processing Of The Glycosylated Precursor, H. Bussey, D. Saville, D. Greene, Donald J. Tipper, Keith A. Bostian Aug 1983

Secretion Of Saccharomyces Cerevisiae Killer Toxin: Processing Of The Glycosylated Precursor, H. Bussey, D. Saville, D. Greene, Donald J. Tipper, Keith A. Bostian

Microbiology and Physiological Systems Publications and Presentations

Killer toxin secretion was blocked at the restrictive temperature in Saccharomyces cerevisiae sec mutants with conditional defects in the S. cerevisiae secretory pathway leading to accumulation of endoplasmic reticulum (sec18), Golgi (sec7), or secretory vesicles (sec1). A 43,000-molecular-weight (43K) glycosylated protoxin was found by pulse-labeling in all sec mutants at the restrictive temperature. In sec18 the protoxin was stable after a chase; but in sec7 and sec1 the protoxin was unstable, and in sec1 11K toxin was detected in cell lysates. The chymotrypsin inhibitor tosyl-l-phenylalanyl chloromethyl ketone (TPCK) blocked toxin secretion in vivo in wild-type cells by inhibiting protoxin ...


A Synthesis Of Sleep In Wild Birds, Charles Amlaner, Nigel Ball Dec 1982

A Synthesis Of Sleep In Wild Birds, Charles Amlaner, Nigel Ball

Charles J. Amlaner

No abstract provided.


Twenty-Four Hour Temperature And Activity Measurements Of Herring Gulls (Larus Argentatus), Charles Amlaner, David Mcfarland Dec 1981

Twenty-Four Hour Temperature And Activity Measurements Of Herring Gulls (Larus Argentatus), Charles Amlaner, David Mcfarland

Charles J. Amlaner

No abstract provided.


Coat Protein Synthesis During Sporulation Of Bacillus Subtilis: Immunological Detection Of Soluble Precursors To The 12,200-Dalton Spore Coat Protein, Robert C. Goldman, Donald J. Tipper Sep 1981

Coat Protein Synthesis During Sporulation Of Bacillus Subtilis: Immunological Detection Of Soluble Precursors To The 12,200-Dalton Spore Coat Protein, Robert C. Goldman, Donald J. Tipper

Microbiology and Physiological Systems Publications and Presentations

Antibody specific to the 12,200-dalton spore coat protein of Bacillus subtilis was used to detect the synthesis of cross-reacting material during sporulation. Cross-reacting protein was first detected by immunoprecipitation after 4 h of development and represented at least 1 to 2% of the total soluble protein synthesis at 5.5 h. A polypeptide of 21,000 daltons was detected in immunoprecipitates by gel electrophoresis. This polypeptide did not accumulate in sporulating cells and was rapidly turned over at the time of coat deposition. In contrast, a 32,000-dalton polypeptide reacted with antibody when unlabeled cell protein was denatured with ...


Comparison Of Various Properties Of Low-Molecular-Weight Proteins From Dormant Spores Of Several Bacillus Species, Katherine Yuan, W. Charles Johnson, Donald J. Tipper, Peter Setlow Jun 1981

Comparison Of Various Properties Of Low-Molecular-Weight Proteins From Dormant Spores Of Several Bacillus Species, Katherine Yuan, W. Charles Johnson, Donald J. Tipper, Peter Setlow

Microbiology and Physiological Systems Publications and Presentations

Several properties of the major proteins degraded during germination of spores of Bacillus cereus, Bacillus megaterium, and Bacillus subtilis have been compared. All of the proteins had low molecular weights (6,000 to 13,000) and lacked cysteine, cystine, and tryptophan. The proteins could be subdivided into two groups: group I (B. megaterium A and C proteins, B. cereus A protein, and B. subtilis alpha and beta proteins) and group II (B. cereus and B. megaterium B proteins and B. subtilis gamma protein). Species in group II had lower levels of (or lacked) the amino acids isoleucine, leucine, methionine, and ...


Acid-Soluble Spore Proteins Of Bacillus Subtilis, W. C. Johnson, Donald J. Tipper Jun 1981

Acid-Soluble Spore Proteins Of Bacillus Subtilis, W. C. Johnson, Donald J. Tipper

Microbiology and Physiological Systems Publications and Presentations

Acid-soluble spore proteins (ASSPs) comprise about 5% of the total protein of mature spores of different Bacillus subtilis strains. They consist of three abundant species, alpha, beta, and gamma, four less abundant species, and several minor species, alpha, beta, and gamma make up about 18, 18 and 36%, respectively, of the total ASSPs of strain 168, have molecular weights of 5,900, 5,9000, and 11,000, respectively, and resemble the major (A, C, and B) components of Bacillus megaterium ASSPs in several respects, including sensitivity to a specific B. megaterium spore endopeptidase. However, they have pI's of 6 ...


Sleep In The Herring Gull (Larus Argentatus), Charles Amlaner, David Mcfarland Apr 1981

Sleep In The Herring Gull (Larus Argentatus), Charles Amlaner, David Mcfarland

Charles J. Amlaner

Sleep postures and eye state of free-ranging herring gulls (Larus argentatus) were studied during the breeding season. Three mutually exclusive behaviours were observed, namely sleep, rest-sleep and rest postures. Arousal thresholds, eye blink rates and eye closure time were obtained during these behaviours. Significant relationships existed between eye blinking, eye closure, and a raised threshold of arousal when birds were in the sleep and rest-sleep postures. During a natural disturbance, birds in the sleep posture remained in this posture but did not blink their eyes: this is called pseudo sleep. Male gulls also exhibited a lower threshold of arousal while ...


Listening In To Wildlife, Charles Amlaner, David Macdonald Dec 1980

Listening In To Wildlife, Charles Amlaner, David Macdonald

Charles J. Amlaner

No abstract provided.


Encapsidation Of Yeast Killer Double-Stranded Ribonucleic Acids: Dependence Of M On L, Keith A. Bostian, Joyce A. Sturgeon, Donald J. Tipper Jul 1980

Encapsidation Of Yeast Killer Double-Stranded Ribonucleic Acids: Dependence Of M On L, Keith A. Bostian, Joyce A. Sturgeon, Donald J. Tipper

Microbiology and Physiological Systems Publications and Presentations

Virus-like particles containing either L or M double-stranded ribonucleic acid (dsRNA) were isolated from a killer toxin-producing strains of Saccharomyces cerevisiae (K+ R+). At least 95% of M- and 87% of L-dsRNA were recovered in virus-like particle-containing fractions. The major capsid polypeptides (ScV-P1) of both L and M virus-like particles were shown to be identical, and 95% of the cellular ScV-P1 was found in the virus-like particle-containing fractions. Since L-dsRNA encodes ScV-P1, provision of this protein for encapsidation of M-dsRNA defines at least one functional relationship between these dsRNA genomes and associates the L-dsRNA with the killer character. If encapsidation ...


Nonenzymatic Glycosylation Of Erythrocyte Membrane Proteins. Relevance To Diabetes, J A. Miller, Ellen M. Gravallese, H F. Bunn Apr 1980

Nonenzymatic Glycosylation Of Erythrocyte Membrane Proteins. Relevance To Diabetes, J A. Miller, Ellen M. Gravallese, H F. Bunn

Rheumatology Publications and Presentations

Nonenzymatic glycosylation of proteins of the erythrocyte membrane was determined by incubating erythrocyte ghosts with [3H]borohydride. The incorporation of tritium into protein provides a reliable assay of ketoamine linkages. The membrane proteins from 18 patients with diabetes incorporated twice as much radioactivity as membrane proteins from normal erythrocytes. After acid hydrolysis, amino acid analysis showed that the majority of radioactivity was localized to glucosyllysine. Autoradiograms showed that all of the major proteins of the erythrocyte membrane, separated by electrophoresis on sodium dodecyl sulfate gels, contained ketoamine linkages. No protein bands in either normal or diabetic erythrocytes showed significant preferential ...


Techniques Of Study In Animal Behaviour, Charles Amlaner Dec 1979

Techniques Of Study In Animal Behaviour, Charles Amlaner

Charles J. Amlaner

No abstract provided.


A Handbook On Biotelemetry And Radio Tracking, Charles Amlaner (Editor), David Macdonald (Editor) Dec 1979

A Handbook On Biotelemetry And Radio Tracking, Charles Amlaner (Editor), David Macdonald (Editor)

Charles J. Amlaner

Proceedings of an International Conference on Telemetry and Radio Tracking in Biology and Medicine, held in Oxford, March 20-22, 1979.


Changing Heart Rates Of Herring Gulls When Approached By Humans, Nigel Ball, Charles Amlaner Dec 1979

Changing Heart Rates Of Herring Gulls When Approached By Humans, Nigel Ball, Charles Amlaner

Charles J. Amlaner

No abstract provided.


Morphology And Patterns Of Protein Synthesis During Sporulation Of Bacillus Subtilis Eryr Spo(Ts) Mutants, Robert C. Goldman, Donald J. Tipper May 1979

Morphology And Patterns Of Protein Synthesis During Sporulation Of Bacillus Subtilis Eryr Spo(Ts) Mutants, Robert C. Goldman, Donald J. Tipper

Microbiology and Physiological Systems Publications and Presentations

Erythromycin-resistant (Eryr) mutants of Bacillus subtilis 168 fail to sporulate at high temperature (47 degrees C) but sporulate normally at 30 to 35 degrees C. They also fail to sporulate at any temperature in the presence of 2.5 micrograms of erythromycin per ml. Neither of these nonpermissive conditions appears to affect vegetative growth, and the periods of sensitivity to both conditions extend from 40 to 90% of the sporulation period. At 47 degrees C, net incorporation of methionine and phenylalanine in postexponential Eryr and 168 cells was similar, and fractionation of the labeled products by polyacrylamide gel electrophoresis gave ...


Location Of Peptidoglycan Lytic Enzymes In Bacillus Sphaericus, M. Guinand, M. J. Vacheron, G. Michel, Donald J. Tipper Apr 1979

Location Of Peptidoglycan Lytic Enzymes In Bacillus Sphaericus, M. Guinand, M. J. Vacheron, G. Michel, Donald J. Tipper

Microbiology and Physiological Systems Publications and Presentations

The level of three peptidoglycan hydrolases was determined in the mother cell compartment and forespores of Bacillus sphaericus. Vegetative and sporulating cells contained in LD-carboxypeptidase active only on the vegetative cell wall peptidoglycan, and we have previously shown that sporulation is accompanied by the production of two new enzymes active only on the spore cortex peptidoglycan. These gamma-D-glutamyl-meso-diaminopimelate endopeptidase and a meso-diaminopimelate-D-alanine dipeptidase. The LD-carboxypeptidase activity appeared to be located in the membranes of both the mother cells and forespores. Endopeptidase activity was located in the integument fraction of the forespores, and the dipeptidase activity was only found in the ...