Open Access. Powered by Scholars. Published by Universities.®

Biochemistry, Biophysics, and Structural Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 5 of 5

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Nonenzymatic Glycosylation Of Erythrocyte Membrane Proteins. Relevance To Diabetes, J A. Miller, Ellen M. Gravallese, H F. Bunn Apr 2015

Nonenzymatic Glycosylation Of Erythrocyte Membrane Proteins. Relevance To Diabetes, J A. Miller, Ellen M. Gravallese, H F. Bunn

Ellen M. Gravallese

Nonenzymatic glycosylation of proteins of the erythrocyte membrane was determined by incubating erythrocyte ghosts with [3H]borohydride. The incorporation of tritium into protein provides a reliable assay of ketoamine linkages. The membrane proteins from 18 patients with diabetes incorporated twice as much radioactivity as membrane proteins from normal erythrocytes. After acid hydrolysis, amino acid analysis showed that the majority of radioactivity was localized to glucosyllysine. Autoradiograms showed that all of the major proteins of the erythrocyte membrane, separated by electrophoresis on sodium dodecyl sulfate gels, contained ketoamine linkages. No protein bands in either normal or diabetic erythrocytes showed significant preferential ...


Distribution Of Poly(Adp-Ribose) Glycohydrolase In Different Functional Domains Of The Cell Nucleus, Gustavo Pacheco-Rodriguez B.S., M.S. Aug 1996

Distribution Of Poly(Adp-Ribose) Glycohydrolase In Different Functional Domains Of The Cell Nucleus, Gustavo Pacheco-Rodriguez B.S., M.S.

Theses and Dissertations

Pacheco-Rodriguez, Gustavo, Distribution of Poly(ADP-ribose) Glycohydrolase in Different Functional Domains of the Cell Nucleus. Doctor of Philosophy (Biomedical Sciences), August, 1996, 147 pp, 3 tables, 44 illustrations, bibliography, 138 titles. In this study, the distributor poly(ADP-ribose) glycohydrolase (PARG) in different subdomains of the cell nucleus and the role of non-covalent interactions of poly(ADP-ribose) with nuclear proteins have been characterized. An assay that allows the simultaneous determination of specific non-covalent interactions of poly (ADP-ribose) with nuclear proteins as well as PARG activity by high resolution polyacrylamide gel electrophoresis was developed. This method was made possible by the enzymatic ...


Coat Protein Synthesis During Sporulation Of Bacillus Subtilis: Immunological Detection Of Soluble Precursors To The 12,200-Dalton Spore Coat Protein, Robert C. Goldman, Donald J. Tipper Sep 1981

Coat Protein Synthesis During Sporulation Of Bacillus Subtilis: Immunological Detection Of Soluble Precursors To The 12,200-Dalton Spore Coat Protein, Robert C. Goldman, Donald J. Tipper

Microbiology and Physiological Systems Publications and Presentations

Antibody specific to the 12,200-dalton spore coat protein of Bacillus subtilis was used to detect the synthesis of cross-reacting material during sporulation. Cross-reacting protein was first detected by immunoprecipitation after 4 h of development and represented at least 1 to 2% of the total soluble protein synthesis at 5.5 h. A polypeptide of 21,000 daltons was detected in immunoprecipitates by gel electrophoresis. This polypeptide did not accumulate in sporulating cells and was rapidly turned over at the time of coat deposition. In contrast, a 32,000-dalton polypeptide reacted with antibody when unlabeled cell protein was denatured with ...


Comparison Of Various Properties Of Low-Molecular-Weight Proteins From Dormant Spores Of Several Bacillus Species, Katherine Yuan, W. Charles Johnson, Donald J. Tipper, Peter Setlow Jun 1981

Comparison Of Various Properties Of Low-Molecular-Weight Proteins From Dormant Spores Of Several Bacillus Species, Katherine Yuan, W. Charles Johnson, Donald J. Tipper, Peter Setlow

Microbiology and Physiological Systems Publications and Presentations

Several properties of the major proteins degraded during germination of spores of Bacillus cereus, Bacillus megaterium, and Bacillus subtilis have been compared. All of the proteins had low molecular weights (6,000 to 13,000) and lacked cysteine, cystine, and tryptophan. The proteins could be subdivided into two groups: group I (B. megaterium A and C proteins, B. cereus A protein, and B. subtilis alpha and beta proteins) and group II (B. cereus and B. megaterium B proteins and B. subtilis gamma protein). Species in group II had lower levels of (or lacked) the amino acids isoleucine, leucine, methionine, and ...


Nonenzymatic Glycosylation Of Erythrocyte Membrane Proteins. Relevance To Diabetes, J A. Miller, Ellen M. Gravallese, H F. Bunn Apr 1980

Nonenzymatic Glycosylation Of Erythrocyte Membrane Proteins. Relevance To Diabetes, J A. Miller, Ellen M. Gravallese, H F. Bunn

Rheumatology Publications and Presentations

Nonenzymatic glycosylation of proteins of the erythrocyte membrane was determined by incubating erythrocyte ghosts with [3H]borohydride. The incorporation of tritium into protein provides a reliable assay of ketoamine linkages. The membrane proteins from 18 patients with diabetes incorporated twice as much radioactivity as membrane proteins from normal erythrocytes. After acid hydrolysis, amino acid analysis showed that the majority of radioactivity was localized to glucosyllysine. Autoradiograms showed that all of the major proteins of the erythrocyte membrane, separated by electrophoresis on sodium dodecyl sulfate gels, contained ketoamine linkages. No protein bands in either normal or diabetic erythrocytes showed significant preferential ...