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Biochemistry, Biophysics, and Structural Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Physiology

2015

University of Pennsylvania

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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Spontaneous Pancreatitis Caused By Tissue-Specific Gene Ablation Of Hhex In Mice, Mark Ferreira Jan 2015

Spontaneous Pancreatitis Caused By Tissue-Specific Gene Ablation Of Hhex In Mice, Mark Ferreira

Publicly Accessible Penn Dissertations

Background & Aims: Perturbations in pancreatic ductal bicarbonate secretion often result in chronic pancreatitis. Although the physiological mechanism of ductal secretion is known, its transcriptional control is not well characterized. Here, we investigate the role of the transcription factor Hematopoietically-expressed homeobox protein (Hhex) in pancreatic secretion and pancreatitis.

Methods: We derived mice with pancreas-specific, Cre-mediated Hhex gene ablation to determine the requirement of Hhex in the pancreatic duct in early life and in adult stages. Histological and immunostaining analyses were used to detect the presence of pathology. Pancreatic primary ductal cells (PDCs) were isolated to discover differentially expressed transcripts upon acute ...