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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Coat Protein Synthesis During Sporulation Of Bacillus Subtilis: Immunological Detection Of Soluble Precursors To The 12,200-Dalton Spore Coat Protein, Robert C. Goldman, Donald J. Tipper Sep 1981

Coat Protein Synthesis During Sporulation Of Bacillus Subtilis: Immunological Detection Of Soluble Precursors To The 12,200-Dalton Spore Coat Protein, Robert C. Goldman, Donald J. Tipper

Microbiology and Physiological Systems Publications and Presentations

Antibody specific to the 12,200-dalton spore coat protein of Bacillus subtilis was used to detect the synthesis of cross-reacting material during sporulation. Cross-reacting protein was first detected by immunoprecipitation after 4 h of development and represented at least 1 to 2% of the total soluble protein synthesis at 5.5 h. A polypeptide of 21,000 daltons was detected in immunoprecipitates by gel electrophoresis. This polypeptide did not accumulate in sporulating cells and was rapidly turned over at the time of coat deposition. In contrast, a 32,000-dalton polypeptide reacted with antibody when unlabeled cell protein was denatured with ...


Comparison Of Various Properties Of Low-Molecular-Weight Proteins From Dormant Spores Of Several Bacillus Species, Katherine Yuan, W. Charles Johnson, Donald J. Tipper, Peter Setlow Jun 1981

Comparison Of Various Properties Of Low-Molecular-Weight Proteins From Dormant Spores Of Several Bacillus Species, Katherine Yuan, W. Charles Johnson, Donald J. Tipper, Peter Setlow

Microbiology and Physiological Systems Publications and Presentations

Several properties of the major proteins degraded during germination of spores of Bacillus cereus, Bacillus megaterium, and Bacillus subtilis have been compared. All of the proteins had low molecular weights (6,000 to 13,000) and lacked cysteine, cystine, and tryptophan. The proteins could be subdivided into two groups: group I (B. megaterium A and C proteins, B. cereus A protein, and B. subtilis alpha and beta proteins) and group II (B. cereus and B. megaterium B proteins and B. subtilis gamma protein). Species in group II had lower levels of (or lacked) the amino acids isoleucine, leucine, methionine, and ...


Acid-Soluble Spore Proteins Of Bacillus Subtilis, W. C. Johnson, Donald J. Tipper Jun 1981

Acid-Soluble Spore Proteins Of Bacillus Subtilis, W. C. Johnson, Donald J. Tipper

Microbiology and Physiological Systems Publications and Presentations

Acid-soluble spore proteins (ASSPs) comprise about 5% of the total protein of mature spores of different Bacillus subtilis strains. They consist of three abundant species, alpha, beta, and gamma, four less abundant species, and several minor species, alpha, beta, and gamma make up about 18, 18 and 36%, respectively, of the total ASSPs of strain 168, have molecular weights of 5,900, 5,9000, and 11,000, respectively, and resemble the major (A, C, and B) components of Bacillus megaterium ASSPs in several respects, including sensitivity to a specific B. megaterium spore endopeptidase. However, they have pI's of 6 ...


Sleep In The Herring Gull (Larus Argentatus), Charles Amlaner, David Mcfarland Apr 1981

Sleep In The Herring Gull (Larus Argentatus), Charles Amlaner, David Mcfarland

Charles J. Amlaner

Sleep postures and eye state of free-ranging herring gulls (Larus argentatus) were studied during the breeding season. Three mutually exclusive behaviours were observed, namely sleep, rest-sleep and rest postures. Arousal thresholds, eye blink rates and eye closure time were obtained during these behaviours. Significant relationships existed between eye blinking, eye closure, and a raised threshold of arousal when birds were in the sleep and rest-sleep postures. During a natural disturbance, birds in the sleep posture remained in this posture but did not blink their eyes: this is called pseudo sleep. Male gulls also exhibited a lower threshold of arousal while ...


Listening In To Wildlife, Charles Amlaner, David Macdonald Dec 1980

Listening In To Wildlife, Charles Amlaner, David Macdonald

Charles J. Amlaner

No abstract provided.