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Biochemistry, Biophysics, and Structural Biology Commons

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Articles 1 - 7 of 7

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Morphology Of An Escherichia Coli Mutant With A Temperature-Dependent Round Cell Shape, Makoto Iwaya, Robert Goldman, Donald J. Tipper, B. Feingold, Jack L. Strominger Dec 1978

Morphology Of An Escherichia Coli Mutant With A Temperature-Dependent Round Cell Shape, Makoto Iwaya, Robert Goldman, Donald J. Tipper, B. Feingold, Jack L. Strominger

Microbiology and Physiological Systems Publications and Presentations

Mutants of Escherichia coli capable of growing in the presence of 10 microgram of mecillinam per ml were selected after intensive mutagenesis. Of these mutants, 1.4% formed normal, rod-shaped cells at 30 degrees C but grew as spherical cells at 42 degrees C. The phenotype of one of these rod(Ts) mutants was 88% cotransducible with lip (14.3 min), and all lip+ rod(Ts) transductants of a lip recipient had the following characteristics: (i) growth was relatively sensitive to mecillinam at 30 degrees C but relatively resistant to mecillinam at 42 degrees C; (ii) penicillin-binding protein 2 was ...


Voltage-Controlled Oscillators: Inexpensive Alternative To Analog-To-Digital Converters, Brian Partridge, Richard Dawkins, Charles Amlaner Sep 1978

Voltage-Controlled Oscillators: Inexpensive Alternative To Analog-To-Digital Converters, Brian Partridge, Richard Dawkins, Charles Amlaner

Charles J. Amlaner

Describes a simple software mimic of an analog-to-digital (A/D) converter that is suitable for most laboratory applications requiring A/D. The routine samples output from a voltage-controlled oscillator that can be built for around $6. Results of proving trials show the output to be linear and sampling rates of up to 50/sec are possible.


Design Of A Remotely Controlled 'Locomotory' Platform Useful In Ethological Studies., Charles Amlaner, Robert Korzyniowski, Robert Bender, John Stout Sep 1978

Design Of A Remotely Controlled 'Locomotory' Platform Useful In Ethological Studies., Charles Amlaner, Robert Korzyniowski, Robert Bender, John Stout

Charles J. Amlaner

A platform capable of movement from one point to another under remote control has been designed for use in behavior studies. Electrical and mechanical design criteria for the platform are discussed, and schematics and engineering drawings are included. The primary application was to study communication between Glaucous-winged gulls. Other applications are suggested.


Bacillus Subtilis Spore Coats: Complexity And Purification Of A Unique Polypeptide Component, Robert C. Goldman, Donald J. Tipper Sep 1978

Bacillus Subtilis Spore Coats: Complexity And Purification Of A Unique Polypeptide Component, Robert C. Goldman, Donald J. Tipper

Microbiology and Physiological Systems Publications and Presentations

Extensively washed, dormant spores of Bacillus subtilis were disrupted with glass beads in buffer at pH 7 in the presence of protease inhibitors. Approximately 31% of the total spore protein was soluble, and another 14% was removed from the insoluble fraction by hydrolysis with lysozyme and washing with 1 M KCl and 0.1% sodium dodecyl sulfate. The residual spore integuments comprised 55% of the total spore proteins and consisted of coats and residual membrane components. Treatment of integuments with sodium dodecyl sulfate and reducing agents at pH 10 solubilized 40% of the total spore protein. Seven low-molecular-weight polypeptide components ...


Aggressive Communication By Larus Glaucescens. Part Vi: Interactions Of Territory Residents With A Remotely Controlled, Locomotory Model, Charles Amlaner Dec 1977

Aggressive Communication By Larus Glaucescens. Part Vi: Interactions Of Territory Residents With A Remotely Controlled, Locomotory Model, Charles Amlaner

Charles J. Amlaner

Observations and experiments on aggressive communication by Larus glaucescens gulls were conducted on Colville Island in 1974. The colony contains approximately 1,800 breeding pairs of Ss. Defenders maintaining a territory utilized defense strategies when responding to a locomotory model. Model directional movement, body posture, and orientation were highly correlated with subsequent behavioral response by the defender. Directional model movement caused an immediate position adjustment by the defender in most cases. The sex of defending birds interacting with the model was not distributed randomly. Males interacted more than females. Evidence supports the observation that male defenders are more aggressive than ...


Effects Of Telemetry Transmitter Weight On Breeding Success In Herring Gulls, Charles Amlaner, Richard Sibly, Robin Mccleery Dec 1977

Effects Of Telemetry Transmitter Weight On Breeding Success In Herring Gulls, Charles Amlaner, Richard Sibly, Robin Mccleery

Charles J. Amlaner

Dummy ratio-tracking transmitters weighing 10, 30 and 50 g were attached to randomly chosen pairs of breeding herring gulls (Larus argentatus). Breeding success was measured as a function of eggs hatched and chicks fledged. Fewer clutches survived if patients were subjected to more severe treatments (p less than 0.02). Component effects (catching pairs of gulls or increased transmitter weight) were not singularly significant in decreasing clutch success.


Biotelemetry From Free Ranging Animals, Charles Amlaner Dec 1977

Biotelemetry From Free Ranging Animals, Charles Amlaner

Charles J. Amlaner

No abstract provided.