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Biochemistry, Biophysics, and Structural Biology Commons

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Physiology

GSBS Student Publications

Chromatin; Chromatin Assembly and Disassembly; Chromatin Immunoprecipitation; Diamide; Gene Expression Profiling; Gene Expression Regulation, Fungal; Genes, Fungal; Histone-Lysine N-Methyltransferase; Histones; Methylation; Nucleosomes; Phosphorylation; Repressor Proteins; Ribosomes; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Sequence Deletion; Stress, Physiological; Substrate Specificity; Time Factors; Time-Lapse Imaging; Transcription, Genetic

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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Systematic Dissection Of Roles For Chromatin Regulators In A Yeast Stress Response, Assaf Weiner, Hsiuyi V. Chen, Chih Long Liu, Ayelet Rahat, Avital Klien, Luis Soares, Mohanram Gudipati, Jenna Pfeffner, Aviv Regev, Stephen Buratowski, Jeffrey A. Pleiss, Nir Friedman, Oliver J. Rando Jul 2012

Systematic Dissection Of Roles For Chromatin Regulators In A Yeast Stress Response, Assaf Weiner, Hsiuyi V. Chen, Chih Long Liu, Ayelet Rahat, Avital Klien, Luis Soares, Mohanram Gudipati, Jenna Pfeffner, Aviv Regev, Stephen Buratowski, Jeffrey A. Pleiss, Nir Friedman, Oliver J. Rando

GSBS Student Publications

Packaging of eukaryotic genomes into chromatin has wide-ranging effects on gene transcription. Curiously, it is commonly observed that deletion of a global chromatin regulator affects expression of only a limited subset of genes bound to or modified by the regulator in question. However, in many single-gene studies it has become clear that chromatin regulators often do not affect steady-state transcription, but instead are required for normal transcriptional reprogramming by environmental cues. We therefore have systematically investigated the effects of 83 histone mutants, and 119 gene deletion mutants, on induction/repression dynamics of 170 transcripts in response to diamide stress in ...