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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Functional Overlap Among Distinct G1/S Inhibitory Pathways Allows Robust G1 Arrest By Yeast Mating Pheromones, Patricia A. Pope, Peter M. Pryciak Dec 2013

Functional Overlap Among Distinct G1/S Inhibitory Pathways Allows Robust G1 Arrest By Yeast Mating Pheromones, Patricia A. Pope, Peter M. Pryciak

GSBS Student Publications

In budding yeast, mating pheromones arrest the cell cycle in G1 phase via a pheromone-activated Cdk-inhibitor (CKI) protein, Far1. Alternate pathways must also exist, however, because deleting the cyclin CLN2 restores pheromone arrest to far1 cells. Here we probe whether these alternate pathways require the G1/S transcriptional repressors Whi5 and Stb1 or the CKI protein Sic1, whose metazoan analogues (Rb or p27) antagonize cell cycle entry. Removing Whi5 and Stb1 allows partial escape from G1 arrest in far1 cln2 cells, along with partial derepression of G1/S genes, which implies a repressor-independent route for inhibiting G1/S transcription. This ...


Crosstalk Between Casein Kinase Ii And Ste20-Related Kinase Nak1, Lubos Cipak, Sneha Gupta, Iva Rajovic, Quan-Wen Jin, Dorothea Anrather, Gustav Ammerer, Dannel Mccollum, Juraj Gregan Mar 2013

Crosstalk Between Casein Kinase Ii And Ste20-Related Kinase Nak1, Lubos Cipak, Sneha Gupta, Iva Rajovic, Quan-Wen Jin, Dorothea Anrather, Gustav Ammerer, Dannel Mccollum, Juraj Gregan

GSBS Student Publications

Although the sterile 20 (Ste20) serine/threonine protein kinase was originally identified as a component of the S. cerevisiae mating pathway, it has homologs in higher eukaryotes and is part of a larger family of Ste20-like kinases. Ste20-like kinases are involved in multiple cellular processes, such as cell growth, morphogenesis, apoptosis and immune response. Carrying out such a diverse array of biological functions requires numerous regulatory inputs and outputs in the form of protein-protein interactions and post-translational modifications. Hence, a thorough knowledge of Ste20-like kinase binding partners and phosphorylation sites will be essential for understanding the various roles of these ...