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Biochemistry, Biophysics, and Structural Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Molecular Biology

Cellular

2005

Articles 1 - 3 of 3

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Analysis Of A Nuclear Complex Of Proteins That Form A Spindle Matrix During Mitosis In Drosophila , Uttama Rath Jan 2005

Analysis Of A Nuclear Complex Of Proteins That Form A Spindle Matrix During Mitosis In Drosophila , Uttama Rath

Retrospective Theses and Dissertations

A spindle matrix is a macromolecular structure hypothesized to provide a stationary support or strut for the anchorage of motor proteins during force generation, microtubule sliding, and chromosome segregation in mitosis. Previous work in Drosophila has shown Skeletor, as the first putative molecular candidate of spindle matrix structure since it possesses many features consistent with the proposed spindle matrix structure.;We have identified two more putative spindle matrix candidate proteins, Chromator and Megator, using yeast two-hybrid interaction assay, cross-immunoprecipitation and immunocytochemistry. Chromator is a novel and essential chromodomain containing protein that interacts directly with Skeletor and extensively colocalizes with it ...


Genetic Profiling Of Mycoplasma Hyopneumoniae , Melissa L. Madsen Jan 2005

Genetic Profiling Of Mycoplasma Hyopneumoniae , Melissa L. Madsen

Retrospective Theses and Dissertations

A microarray was constructed and applied to transcriptional profiling and genetic variation studies of Mycoplasma hyopneumoniae. The genome sequence enabled the construction of the microarray to allow a global approach to understanding fundamental processes in M. hyopneumoniae . These studies focused on whether M. hyopneumoniae regulates its genes under different environmental conditions and if genetic changes can be correlated with virulence. The microarray consisted of 632 open reading frames represented by polymerase chain reaction products and were used in a two-color experimental design. Data were analyzed using a mixed linear statistical model. Unique features implemented in these studies included the printing ...


Functional Analysis Of The Crinkly4 Gene Family In Arabidopsis Thaliana And Viviparous1 Promoter In Zea Mays , Xueyuan Cao Jan 2005

Functional Analysis Of The Crinkly4 Gene Family In Arabidopsis Thaliana And Viviparous1 Promoter In Zea Mays , Xueyuan Cao

Retrospective Theses and Dissertations

The cereal endosperm is an excellent system to study cell differentiation and fate specification. The aleurone of maize is one cell layer and requires continuous positional cues to specify and maintain its identity. A genetic hierarchy is implicated in this process. CRINKLY4 ( Cr4), a receptor-like kinase, belongs to cell fate-specifying genes and is required to specify aleurone cell identity. Viviparous1 ( Vp1), a B3 domain-containing transcription factor, functions late in development to regulate the expression of C1 in aleurone cells. It also plays an important regulatory role in late embryogenesis and seed maturation with abscisic acid (ABA).;The Arabidopsis genome encodes ...