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Biochemistry, Biophysics, and Structural Biology Commons

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Molecular Biology

Botany (Physiology and molecular biology)

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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Expression Of Genes Encoding Acetyl-Coa Carboxylase, Biotin Synthase, And Acetyl-Coa Generating Enzymes In Arabidopsis Thaliana , Jinshan Ke Jan 1997

Expression Of Genes Encoding Acetyl-Coa Carboxylase, Biotin Synthase, And Acetyl-Coa Generating Enzymes In Arabidopsis Thaliana , Jinshan Ke

Retrospective Theses and Dissertations

Acetyl-CoA carboxylase catalyzes the formation of malonyl-CoA from acetyl-CoA. The four genes encoding heteromeric acetyl-CoA carboxylase are coordinately expressed in leaves, flowers, and developing siliques and embryos. The highest expression occurs in tissues undergoing rapid growth and high oil deposition. The gene encoding the E1 [beta] subunit of chloroplastidic pyruvate dehydrogenase has an identical pattern, indicating it may provide acetyl-CoA for seeds. In contrast, the gene encoding acetyl-CoA synthetase has a different expression pattern and is expressed at low levels in seeds;The ACC1 genes encoding homomeric acetyl-CoA shows transient expression in a variety of cell types. The homomeric acetyl-CoA ...


Expression Of Chlamydomonas Reinhardtii Periplasmic Carbonic Anhydrase In Escherichia Coli And Transgenic Tobacco Plants , Cyril Sebastian Roberts Jan 1994

Expression Of Chlamydomonas Reinhardtii Periplasmic Carbonic Anhydrase In Escherichia Coli And Transgenic Tobacco Plants , Cyril Sebastian Roberts

Retrospective Theses and Dissertations

Carbonic anhydrase (CA) catalyzes the reversible hydration of CO2. The periplasmic CA gene CAH1 of Chlamydomonas reinhardtii codes for a highly processed secreted glycoprotein. The primary translation product of the CAH1 gene is targeted to the algae's ER, where it is proteolytically processed to yield two different subunits, glycosylated, assembled into an active heterotetramer, and secreted. Expression of CAH1 in Escherichia coli and tobacco plants were effected in this study. For bacterial expression, two constructs, one with and another without, the algal target leader sequence were used. Similarly, after replacing the algal's target leader sequence with that from ...


Molecular And Ultrastructural Aspects Of The Adaptation Of Chlamydomonas Reinhardtii To Limiting Co2 , Anne Margaret Geraghty Jan 1994

Molecular And Ultrastructural Aspects Of The Adaptation Of Chlamydomonas Reinhardtii To Limiting Co2 , Anne Margaret Geraghty

Retrospective Theses and Dissertations

Change occurring in Chlamydomonas reinhardtii as cells adapt to limiting CO2 conditions were examined at both a molecular level and an ultrastructural level. It was demonstrated using antibodies that the time course of the de novo synthesis of LIP-21, a 21 kD limiting-CO2 induced polypeptide, correlates with the appearance of CO2 concentrating activity and that expression of the nuclear gene for LIP-21 is regulated at the level of translatable message. These results are consistent with LIP-21 being a component of an adaptive response to limiting CO2, such as the induction of the CO2 concentrating mechanism. Phase separation of membrane proteins ...


Changes In Gene Expression During Induction Of The Carbon Dioxide Concentrating Mechanism In Chlamydomonas Reinhardtii , Thomas Leslie Winder Jan 1991

Changes In Gene Expression During Induction Of The Carbon Dioxide Concentrating Mechanism In Chlamydomonas Reinhardtii , Thomas Leslie Winder

Retrospective Theses and Dissertations

The regulation of genes by CO[subscript]2 concentration in wild type and mutants of the unicellular green alga Chlamydomonas reinhardtii was studied. This and other algal species can photosynthesize efficiently under limiting concentrations of CO[subscript]2 due to the activities of a CO[subscript]2 concentrating mechanism and carbonic anhydrase. A cDNA copy of the gene that codes for a periplasmic carbonic anhydrase (perCA) was isolated and used to study regulation of perCA synthesis in a cell wall-deficient mutant (CW-15). Over-expression of perCA in CW-15, relative to wild type, was found at the levels of enzyme activity, protein ...