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3' Untranslated Regions; Animals; *Caenorhabditis elegans; Caenorhabditis elegans Proteins; Carrier Proteins; Gene Expression Profiling; *Gene Expression Regulation, Developmental; MicroRNAs; Oligonucleotide Array Sequence Analysis; RNA, Messenger; Recombinant Fusion Proteins
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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Systematic Analysis Of Dynamic Mirna-Target Interactions During C. Elegans Development, Liang Zhang, Molly Hammell, Brian A. Kudlow, Victor R. Ambros, Min Han
Systematic Analysis Of Dynamic Mirna-Target Interactions During C. Elegans Development, Liang Zhang, Molly Hammell, Brian A. Kudlow, Victor R. Ambros, Min Han
Program in Molecular Medicine Publications and Presentations
Although microRNA (miRNA)-mediated functions have been implicated in many aspects of animal development, the majority of miRNA::mRNA regulatory interactions remain to be characterized experimentally. We used an AIN/GW182 protein immunoprecipitation approach to systematically analyze miRNA::mRNA interactions during C. elegans development. We characterized the composition of miRNAs in functional miRNA-induced silencing complexes (miRISCs) at each developmental stage and identified three sets of miRNAs with distinct stage-specificity of function. We then identified thousands of miRNA targets in each developmental stage, including a significant portion that is subject to differential miRNA regulation during development. By identifying thousands of miRNA ...