Open Access. Powered by Scholars. Published by Universities.®

Biochemistry, Biophysics, and Structural Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Molecular Biology

2009

Series

Genetics, Development and Cell Biology Publications

Articles 1 - 1 of 1

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

An Affinity-Based Scoring Scheme For Predicting Dna-Binding Activities Of Modularly Assembled Zinc-Finger Proteins, Jeffry D. Sander, Peter Zaback, J. Keith Joung, Daniel F. Voytas, Drena L. Dobbs Jan 2009

An Affinity-Based Scoring Scheme For Predicting Dna-Binding Activities Of Modularly Assembled Zinc-Finger Proteins, Jeffry D. Sander, Peter Zaback, J. Keith Joung, Daniel F. Voytas, Drena L. Dobbs

Genetics, Development and Cell Biology Publications

Zinc-finger proteins (ZFPs) have long been recognized for their potential to manipulate genetic information because they can be engineered to bind novel DNA targets. Individual zinc-finger domains (ZFDs) bind specific DNA triplet sequences; their apparent modularity has led some groups to propose methods that allow virtually any desired DNA motif to be targeted in vitro. In practice, however, ZFPs engineered using this ‘modular assembly’ approach do not always function well in vivo. Here we report a modular assembly scoring strategy that both identifies combinations of modules least likely to function efficientlyin vivo and provides accurate estimates of their relative ...