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Biochemistry, Biophysics, and Structural Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Molecular Biology

1991

University of Massachusetts Medical School

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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Er Translocation Intermediates Are Adjacent To A Nonglycosylated 34-Kd Integral Membrane Protein, Kennan V. Kellaris, Sharon Bowen, Reid Gilmore Jul 1991

Er Translocation Intermediates Are Adjacent To A Nonglycosylated 34-Kd Integral Membrane Protein, Kennan V. Kellaris, Sharon Bowen, Reid Gilmore

Open Access Articles

We have used the homobifunctional cross-linking reagent disuccinimidyl suberate (DSS) to identify proteins that are adjacent to nascent polypeptides undergoing translocations across mammalian rough ER. Translocation intermediates were assembled by supplementing cell free translations of truncated mRNAs with the signal recognition particle (SRP) and microsomal membrane vesicles. Two prominent cross-linked products of 45 and 64 kD were detected. The 64-kD product was obtained when the cell free translation contained SRP, while formation of the 45-kD product required both SRP and translocation competent microsomal membrane vesicles. In agreement with previous investigators, we suggest that the 64-kD product arises by cross-linking of ...