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Molecular Biology

Theses and Dissertations

Regeneration

Articles 1 - 4 of 4

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Extracellular Matrix Remodeling And The Inflammatory Response During Skeletal Muscle Regeneration In Sarcopenic Obese Mice, Lemuel Arthur Brown Dec 2016

Extracellular Matrix Remodeling And The Inflammatory Response During Skeletal Muscle Regeneration In Sarcopenic Obese Mice, Lemuel Arthur Brown

Theses and Dissertations

AIM: Sarocpenic obesity is a national concern within the United States because this metabolic syndrome is tied with reduced mobility and quality of life. Both obesity and aging are associated with insulin-resistance, chronic low-grade inflammation and muscle weakness. Skeletal muscle regeneration is a process that involves the coordinated effort of myogenic regulatory factors (MRFs), inflammatory signaling, and extracellular matrix (ECM) remodeling for optimal regeneration. It has been demonstrated that obesity and aging have a reduction in muscle regeneration. It has not been examined if sarcopenic obesity will further reduce muscle mass and the regenerative process. The purpose of this study ...


Understanding Connexin43 Dependent Skeletal Morphogenesis - Progress Made And Promises Ahead, Joyita Bhadra Jan 2016

Understanding Connexin43 Dependent Skeletal Morphogenesis - Progress Made And Promises Ahead, Joyita Bhadra

Theses and Dissertations

Skeletal morphogenesis is a complex process through which bones grow to their correct size and shape and find their location in the body. A number of signaling pathways are involved in proper regulation of this process. Among them, Gap Junctional Intercellular Communication (GJIC) is important for proper bone growth and patterning. In our lab, we use regenerating caudal fin of a zebrafish to study skeletal morphogenesis. Our examination of a fin length mutant sofb123 has identified a gap junction protein called Connexin43 (Cx43). Cx43 regulates growth and patterning during regeneration by promoting cell proliferation and suppressing joint formation, respectively. The ...


Development Of Peripheral Innervation In The Frog Xenopus Laevis, Mitali A. Gandhi Aug 2014

Development Of Peripheral Innervation In The Frog Xenopus Laevis, Mitali A. Gandhi

Theses and Dissertations

The skin in Xenopus laevis is innervated by two different sets of mechanosensory neurons at different times during development. Rohon Beard (RB) neurons start differentiating during gastrulation, innervate the embryonic skin and mediate sensory function during hatching. Dorsal Root Ganglion (DRG) neurons start differentiating after neural crest migration, innervate adult epidermal targets and mediate mechanosensory function during larval and adult stages and eventually replace RB neurons. The change in sensory neurons occurs during the transformation of skin, sensory structures, and behavior from their embryonic to their larval forms. We hypothesized that developmental changes in either the sensory end organs or ...


Determining How Defects In Connexin 43 Cause Skeletal Diseases, Quynh V. Ton Jan 2014

Determining How Defects In Connexin 43 Cause Skeletal Diseases, Quynh V. Ton

Theses and Dissertations

The zebrafish fin is composed of multiple bony fin rays. Each fin ray is comprised of multiple segments separated by joints. Regulatory mechanisms that control joint morphogenesis and ray segment length in zebrafish fins are not fully understood. We utilize the fin length mutants short fin (sof b123) and another long fin (alf dty86) to provide insight into these processes. The sof b123 mutant has short fins and short segments due to a mutation in the gap junction protein gene connexin43 (cx43). In contrast, the alf dty86 mutant has long fins, long segments, and over-expression of cx43. Thus, the two ...