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Molecular Biology

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Microarray

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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Gene Expression And Physiological Analysis To Study Differences Between Oryza Sativa Cultivars Susceptible And Resistant To Chalky Grain Formation Subjected To High Nighttime Temperatures., Nicholas Lawson May 2016

Gene Expression And Physiological Analysis To Study Differences Between Oryza Sativa Cultivars Susceptible And Resistant To Chalky Grain Formation Subjected To High Nighttime Temperatures., Nicholas Lawson

Theses and Dissertations

Starch composition and grain quality of rice is greatly influenced by genotype and environmental factors. The detrimental effects of high nighttime temperatures on rice yield and quality has recently become apparent, with some of the warmest average nighttime temperatures being recorded in the past few years. One of the most notable effects of this stress, an increase in grain chalk formation, correlates with a decrease in quality. This effect varies greatly between cultivars as some show less temperature-sensitive quality reduction than others. The goal of this research is to elucidate fundamental changes that occur in developing plants and grains as ...


Host-Virus Interactions Of Infectious Laryngotracheitis Virus Infection In Cultured Cells, Jeong Yoon Lee Dec 2011

Host-Virus Interactions Of Infectious Laryngotracheitis Virus Infection In Cultured Cells, Jeong Yoon Lee

Theses and Dissertations

Infectious laryngotracheitis virus (ILTV; Gallid herpesvirus 1) causes upper respiratory diseases in mainly chickens and exhibits 90-100% of high morbidity and up to 70% of mortality, resulting in huge economic losses in the poultry industry worldwide.

To study host-ILTV interactions, the changes in genome-wide gene expressions in response to wild-type and vaccine ILTV infections in primary chicken embryo lung cells were investigated using microarray analysis. Results provide crucial insights into host cell pathogenic and immunogenic responses against wild-type and vaccine ILTV infections. Using microarray method and Ingenuity Pathway Analysis (IPA) bioinformatics tool, 273 and 306 differentially expressed genes were identified ...


Mechanisms Of Human Epithelial Cell Immortalization And P16nk4a Induced Telomere-Independent Sencescence, Benjamin Will Darbro Jan 2007

Mechanisms Of Human Epithelial Cell Immortalization And P16nk4a Induced Telomere-Independent Sencescence, Benjamin Will Darbro

Theses and Dissertations

As human epithelial cells age in culture, protein levels of the tumor suppressor protein p16INK4a continue to increase resulting in growth arrest independent of telomere length. Telomere-independent senescence induced by the p16INK4a/Rb tumor suppressor pathway prevents many epithelial cells from becoming immortalized by telomerase alone. Differences in culture conditions have been hypothesized to modulate both p16INK4a expression and replicative capacity of human epithelial cells; however, the mechanism(s) of p16INK4a regulation under these conditions is unknown.

We have demonstrated that p16INK4a expression is delayed and replicative capacity increased in human keratinocytes grown in co-culture ...