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Biochemistry, Biophysics, and Structural Biology Commons

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Molecular Biology

Theses and Dissertations

University of Iowa

Translesion synthesis

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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Substrate Recognition By The Yeast Rev1 Protein And Dna Polymerase Ζ, Craig A Howell Jan 2008

Substrate Recognition By The Yeast Rev1 Protein And Dna Polymerase Ζ, Craig A Howell

Theses and Dissertations

DNA damage blocks replication by classical DNA polymerases, those that replicate nondamaged DNA during normal DNA replication and repair, by altering the geometry of the DNA. Consequently, translesion synthesis, the replication of damaged DNA, is catalyzed by non-classical DNA polymerases, which are capable of accommodating the inherent distorted geometry of damaged DNA.

The yeast Rev1 protein (Rev1p) specifically catalyzes the incorporation of cytosine opposite template guanine and several types of DNA damage utilizing a unique mechanism of nucleotide selection whereby the sidechain of Arg-324 acts as the template by forming hydrogen bonds with the incoming cytosine. To better understand the ...