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Biochemistry, Biophysics, and Structural Biology Commons

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Molecular Biology

Theses and Dissertations

University of Iowa

Keratinocyte

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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Mechanisms Of Human Epithelial Cell Immortalization And P16nk4a Induced Telomere-Independent Sencescence, Benjamin Will Darbro Jan 2007

Mechanisms Of Human Epithelial Cell Immortalization And P16nk4a Induced Telomere-Independent Sencescence, Benjamin Will Darbro

Theses and Dissertations

As human epithelial cells age in culture, protein levels of the tumor suppressor protein p16INK4a continue to increase resulting in growth arrest independent of telomere length. Telomere-independent senescence induced by the p16INK4a/Rb tumor suppressor pathway prevents many epithelial cells from becoming immortalized by telomerase alone. Differences in culture conditions have been hypothesized to modulate both p16INK4a expression and replicative capacity of human epithelial cells; however, the mechanism(s) of p16INK4a regulation under these conditions is unknown.

We have demonstrated that p16INK4a expression is delayed and replicative capacity increased in human keratinocytes grown in co-culture ...