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Biochemistry, Biophysics, and Structural Biology Commons

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Molecular Biology

Honors Theses

Assumption College

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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Vesicle-Membrane Tethering: The Interaction Of Sec1 And Exocyst Subunit Sec6, Lauren O'Connor Jan 2017

Vesicle-Membrane Tethering: The Interaction Of Sec1 And Exocyst Subunit Sec6, Lauren O'Connor

Honors Theses

The exocyst complex is a multi-subunit tethering complex that is used in the process of exocytosis. There are eight subunits in the complex, and these subunits interact with each other as well as proteins outside of the complex to facilitate membrane fusion. Prior research has shown that the exocyst subunit Sec6 and Sec1 from the Sec1/Munc18 (SM) family, a known regulator of membrane fusion, interact with each other. In this experiment, the goal was to crosslink the two proteins and in turn work to identify the interacting amino acid residues that are responsible for the proteins’ interaction. To do ...