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Biochemistry, Biophysics, and Structural Biology Commons

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Molecular Biology

The Texas Medical Center Library

2012

Genome-wide RNAi screen

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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Functional Analysis Of Drosophila Integrator Complex In Snrna 3' End Processing, Jiandong Chen Dec 2012

Functional Analysis Of Drosophila Integrator Complex In Snrna 3' End Processing, Jiandong Chen

UT GSBS Dissertations and Theses (Open Access)

Uridine-rich small nuclear RNAs (U snRNAs) play essential roles in eukaryotic gene expression by facilitating the removal of introns from mRNA precursors and the processing of the replication-dependent histone pre-mRNAs. Formation of the 3’ end of these snRNAs is carried out by a poorly characterized, twelve-membered protein complex named Integrator Complex.

In the effort to understand Integrator Complex function in the formation of the snRNA 3’ end, we performed a functional RNAi screen in Drosophila S2 cells to identify protein factors required for snRNA 3’ end formation. This screen was conducted by using a fluorescence-based reporter that elicits GFP expression ...