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Biochemistry, Biophysics, and Structural Biology Commons

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Cancer Biology

Carroll College

Publication Year

Articles 1 - 4 of 4

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Detection Of K-Ras Colorectal Point Mutations Using Endonuclease V/Ak16dligase And Ligase Detection Reaction Assays, Laura Mirch Apr 2005

Detection Of K-Ras Colorectal Point Mutations Using Endonuclease V/Ak16dligase And Ligase Detection Reaction Assays, Laura Mirch

Life and Environmental Sciences Undergraduate Theses

This research investigates the benefits of utilizing PCR/Ligase Detection Reaction multiplexing coupled with endonuclease V/AK16D ligase treatment in the detection of known and unknown point mutations within DNA. The research focused specifically on the K-ras gene, implicated in the development of certain cancers including colorectal cancer. Identification of point mutations within this gene could lead to early detection of cancer, as well as identify those with a predisposition to develop cancer. An LDR technique was utilized to identify specific, known mutations within the gene sequence, while the endonuclease/ligase treatment identified unknown mutations within the gene sequence. Capillary ...


The Effect Of Pkc 5 Inhibitors On Pma-Induced Apoptosis In Xenopus Laevis Splenocytes, Annie Crater Apr 2003

The Effect Of Pkc 5 Inhibitors On Pma-Induced Apoptosis In Xenopus Laevis Splenocytes, Annie Crater

Life and Environmental Sciences Undergraduate Theses

Protein kinase C (PKC) controls apoptosis in amphibian systems such as that of the South African clawed frog, Xenopus laevis. The effects of cancer inducing agents like phorbol 12-myristate 13-acetate (PMA) when combined with Ca2+ independent PKC inhibitors on programmed cell death were studied in A laevis splenocytes. Regulation of PMA-activated cells was observed via Annexin V-FITC and propidium iodide assays, flow cytometry, and Western blot analysis. Results indicate the PKC inhibitors reduced both apoptosis and cell growth in PMA-stimulated X. laevis splenocytes compared to PMA alone. These results are valuable to future mammalian cancer research. By identifying the various ...


Immunological And Immunohistochemical Methods Used To Study Chromatin Neoantigens Of Novikoff Hepatoma, Steven Binette, Timothy O'Connor Apr 1980

Immunological And Immunohistochemical Methods Used To Study Chromatin Neoantigens Of Novikoff Hepatoma, Steven Binette, Timothy O'Connor

Life and Environmental Sciences Undergraduate Theses

The micro-complement fixation test was used to assay the potency and specificity of rabbit antisera to chromosomal antigens from Novikoff hepatoma and normal rat liver. The complement fixation titration patterns suggest the presence of only a few species of molecules bearing neochromatin antigens. A immunofluorescent technique was adapted to Novikoff hepatoma cells and resulted in the positive staining of Novikoff chromatin and negligible staining in normal rat liver cells.


The Effects Of 3-Methylcholanthrene On Humoral Antibodies And On Antibody-Producing Cells In The Spleen, Chris Erickson Apr 1972

The Effects Of 3-Methylcholanthrene On Humoral Antibodies And On Antibody-Producing Cells In The Spleen, Chris Erickson

Life and Environmental Sciences Undergraduate Theses

An increasing number of reports during the Dast decade have suggested a positive, though far from perfect, correlation between carcinogenicity and the ability of a side variety of carcinogens to impair the immune status of the host. In addition, those conditions resulting in the suppression of the immune reaction of the host, including the use of immunosuppressive drugs with transplant patients, seem to foster the incidence of cancer [St jernsward, 1969]. Burnet [1961] has suggested that the evolutionary significance of the immune response is that it enables the elimination of mutant somatic cells, which might otherwise form neoplasms. Then, possibly ...