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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Electrically Mediated Delivery Of Vector Plasmid Dna Elicits An Antitumor Effect, L. Heller, D. Coppola Oct 2002

Electrically Mediated Delivery Of Vector Plasmid Dna Elicits An Antitumor Effect, L. Heller, D. Coppola

Bioelectrics Publications

In vivo electroporation is an efficient means of increasing plasmid DNA delivery to normal tissues, such as skin and muscle, as well as directly to tumors. In the experiments described here, plasmid DNA was delivered by in vivo electroporation to B16 mouse melanomas using two very different pulsing protocols. Reporter expression increased 21- or 42-fold, respectively with electroporation over injection alone. The growth of experimental melanomas with an approximate diameter of 4 mm on the day of treatment was monitored after electroporation delivery of reporter plasmid DNA. Remarkably, short-term complete regressions using one of these pulsing protocols occurred in up ...


Bioinformatics Support Of Genome Sequencing Projects, Xiaoqiu Huang Jan 2002

Bioinformatics Support Of Genome Sequencing Projects, Xiaoqiu Huang

Computer Science Publications

The genome of an organism is the "book of life". It encodes the complete set of genetic instructions for the development of the organism. The structure of a genome is a linear sequence of nucleotides. Determination of the sequence of a genome lays the foundation for understanding biology at the molecular level. With the current biotechnology, it is a challenging task to determine the sequence of a genome. A sequencing machine can read the sequence of a piece of DNA for up to 1000 bp (base pairs). However, genomes are very huge. For example, the genome of the bacterium E ...


The Atp:Co(I)Rrinoid Adenosyltransferase (Coba) Enzyme Of Salmonella Enterica Requires The 2’-Oh Group Of Atp For Function And Yields Inorganic Triphosphate As Its Reaction Byproduct, Maris V. Fonseca, Nicole R. Baun, Alexander R. Horswill, Ivan Rayment, Jorge C. Escalante-Semerena Jan 2002

The Atp:Co(I)Rrinoid Adenosyltransferase (Coba) Enzyme Of Salmonella Enterica Requires The 2’-Oh Group Of Atp For Function And Yields Inorganic Triphosphate As Its Reaction Byproduct, Maris V. Fonseca, Nicole R. Baun, Alexander R. Horswill, Ivan Rayment, Jorge C. Escalante-Semerena

Biochemistry -- Faculty Publications

The specificity of the ATP:corrinoid adenosyltransferase

(CobA) enzyme of Salmonella enterica serovar

Typhimurium LT2 for its nucleotide substrate was

tested using ATP analogs and alternative nucleotide donors.

The enzyme showed broad specificity for the nucleotide

base and required the 2-OH group of the ribosyl

moiety of ATP for activity. 31P NMR spectroscopy

was used to identify inorganic triphosphate (PPPi) as

the byproduct of the reaction catalyzed by the CobA

enzyme. Cleavage of triphosphate into pyrophosphate

and orthophosphate did not occur, indicating that

triphosphate cleavage was not required for release of

the adenosylcorrinoid product. Triphosphate was a ...