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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Confocal Laser Scanning Microscopy: A Flexible Tool For Polarized Light And Three-Dimensional Fluorescence Imaging Of Compact Bone, Corey Maggiano, Tosha Dupras, Michael Schultz, John Biggerstaff Sep 2009

Confocal Laser Scanning Microscopy: A Flexible Tool For Polarized Light And Three-Dimensional Fluorescence Imaging Of Compact Bone, Corey Maggiano, Tosha Dupras, Michael Schultz, John Biggerstaff

John Biggerstaff

Wide-field polarized light and epifluorescence microscopy have been used to enhance analysis of archaeological bone tissue, providing information on bone formation, modeling, pathology, preservation, age estimation, and biomechanics. Though valuable, these techniques are limited by their inability to remove out-of-focus light and view multiple levels of a sample, restricting our understanding of the three-dimensional (3-D) microarchitecture of compact bone. Modern technological advances, such as microscopic computerized tomography, allow increasing resolution in 3-D bone imaging, but do not allow fluorescence labeling or polarized-light analysis. Confocal laser scanning microscopy (CLSM) is a valuable tool for 3-D histology. However, its application to the ...


Synthesis, Biological Evaluation And Radiochemical Labeling Of A Dansylhydrazone Derivative As A Potential Imaging Agent For Apoptosis, Wenbin Zeng, Min-Liang Yao, David Townsend, George Kabalka, Jonathan Wall, Michael Le Puil, John Biggerstaff, Weimin Miao Jun 2008

Synthesis, Biological Evaluation And Radiochemical Labeling Of A Dansylhydrazone Derivative As A Potential Imaging Agent For Apoptosis, Wenbin Zeng, Min-Liang Yao, David Townsend, George Kabalka, Jonathan Wall, Michael Le Puil, John Biggerstaff, Weimin Miao

John Biggerstaff

To develop a small molecule-based tracer for in vivo apoptosis imaging, dansylhydrazone (DFNSH) was synthesized in 93% yield in less than 30 min. The biological evaluation showed that DFNSH selectively binds to paclitaxel-induced apoptotic cancer cells. The high magnification fluorescent images demonstrate that DFNSH is localized within the cytoplasm of cells that bound Alexa® 488 labeled annexin V on the plasma membrane. [18F]-DFNSH ([18F]-3) was synthesized and isolated in 50–60% radiochemical yields, based on [K/K222]18F, with a synthesis time of 50 min (EOB). The straightforward preparation of fluorine-18 labeled 3 makes it a promising tracer ...


Soluble Fibrin Inhibits Lymphocyte Adherence And Cytotoxicity Against Tumor Cells: Implications For Cancer Metastasis And Immunotherapy, John Biggerstaff, Brandy Weidow, Judith Dexheimer, Gary Warnes, Jacqueline Vidosh, Shonak Patel, Michael Newman, Pretesh Patel Mar 2008

Soluble Fibrin Inhibits Lymphocyte Adherence And Cytotoxicity Against Tumor Cells: Implications For Cancer Metastasis And Immunotherapy, John Biggerstaff, Brandy Weidow, Judith Dexheimer, Gary Warnes, Jacqueline Vidosh, Shonak Patel, Michael Newman, Pretesh Patel

John Biggerstaff

Circulating soluble fibrin (sFn) is elevated in many cancer patients. It is a marker for ongoing disseminated intravascular coagulation and may have prognostic significance. We have demonstrated that sFn inhibited monocyte adherence and cytotoxicity by a mechanism involving blockade of monocyte αMβ2 and tumor cell CD54. It was, therefore, hypothesized that sFn also inhibits lymphocyte and interleukin-2—activated lymphocyte (LAK) adherence and cytotoxicity against tumor cells. This study sought to identify the lymphocyte subset responsible for adherence and killing of A375 melanoma cells and whether sFn inhibited these parameters. Lymphocyte and LAK cell adherence and cytotoxicity, which was adherence dependent ...


Micropropagation Of Field-Grown Perennial Teosinte From Node Culture, J. Zale, L. Ohnoutková, D. West, C. Sams, John Biggerstaff, M. Le Puil Dec 2007

Micropropagation Of Field-Grown Perennial Teosinte From Node Culture, J. Zale, L. Ohnoutková, D. West, C. Sams, John Biggerstaff, M. Le Puil

John Biggerstaff

Propagation of perennial diploid teosinte, Zea diploperennis Iltis, Doebley and Guzmán (2n = 2x = 20), and tetraploid teosinte, Z. perennis (Hitchc.) Reeves and Magelsdorf (2n = 4x = 40), is limited by seed availability. For plants grown in the field, a photoperiod response delays flowering until fall, and seeds may not reach maturity. The objective of this study was to develop an in vitro micropropagation protocol for field grown teosinte. Shoot proliferation was induced from nodes split longitudinally and plated on Murashige and Skoog's (MS) medium supplemented with 5 μM 6-benzyl amino purine and 3% (w/v) sucrose. Both species possess a ...


Soluble Fibrin Inhibits Monocyte Adherence And Cytotoxicity Against Tumor Cells: Implications For Cancer Metastasis, John Biggerstaff, Brandy Weidow, Jacqueline Vidosh, Judith Dexheimer, Shonak Patel, Pretesh Patel Dec 2005

Soluble Fibrin Inhibits Monocyte Adherence And Cytotoxicity Against Tumor Cells: Implications For Cancer Metastasis, John Biggerstaff, Brandy Weidow, Jacqueline Vidosh, Judith Dexheimer, Shonak Patel, Pretesh Patel

John Biggerstaff

Background Soluble fibrin (sFn) is a marker for disseminated intravascular coagulation and may have prognostic significance, especially in metastasis. However, a role for sFn in the etiology of metastatic cancer growth has not been extensively studied. We have reported that sFn cross-linked platelet binding to tumor cells via the major platelet fibrin receptor αIIbβ3, and tumor cell CD54 (ICAM-1), which is the receptor for two of the leukocyte β2 integrins (αLβ2 and aMβ2). We hypothesized that sFn may also affect leukocyte adherence, recognition, and killing of tumor cells. Furthermore, in a rat experimental metastasis model sFn pre-treatment of tumor cells ...