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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Using Golden Gate Assembly To Rapidly Create Genetic Constructs For Transgenic Plants And Promoter Analysis, Jonathan Smith Apr 2015

Using Golden Gate Assembly To Rapidly Create Genetic Constructs For Transgenic Plants And Promoter Analysis, Jonathan Smith

Honors Theses

Golden Gate Assembly, a genetic assembly method in which the ligation and digestion of oligonucleotides occurs in a single step, can be used for an array of purposes. In order to test and take advantage of this assembly method our lab selected three experiments in which it could be used. These included an oxidative stress sensitive green florescent protein, a plant specific form of Golden Gate Assembly coined "Green Gate Assembly," and a viral promoter verification system called "pClone Red."

Arabidopsis (Arabidopsis thaliana) plants were analyzed for the presence of a redox-sensing green fluorescent protein (reduction-oxidation-sensitive green fluorescent protein [roGFP ...


Biochemical Conversion Of Biomass Into Butanol Using Clostridium Acetobutylicum, Eric Stevens Jan 2015

Biochemical Conversion Of Biomass Into Butanol Using Clostridium Acetobutylicum, Eric Stevens

Honors Theses

Butanol is a potential transportation fuel which could eventually replace fossil- derived petroleum and transition the world towards a more sustainable future. This fuel can be produced by enzymatically hydrolyzing pretreated lignocellulosic biomass, and simultaneously fermenting the resultant sugars with specific bacterial strains. In this experiment switchgrass was chemically pretreated in a 75% (v/v) ethanol and 1% H2SO4 (1% v/v) solution and subjected to simultaneous saccharification and fermentation (SSF) with hydrolytic enzymes and Clostridium acetobutylicum. The pH control method for the SSF apparatus was varied between a 50 mM acetate buffer solution (HOAc), and the addition of calcium ...


Deregulation Of Fatty Acid Transport In Escherichia Coli For Enhanced Control Of Biodegradable Plastic Copolymer Production, Ryan A. Scheel May 2014

Deregulation Of Fatty Acid Transport In Escherichia Coli For Enhanced Control Of Biodegradable Plastic Copolymer Production, Ryan A. Scheel

Honors Theses

Polyhydroxyalkanoates (PHA) are one possible alternative for petroleum-based plastics that have been shown to be produced effectively in bacterial systems utilizing fatty acids. The global regulatory genes arcA and ompR are known to regulate steps involved in fatty acid transport and metabolism, making them promising candidates for research. In this study, we deleted the arcA and ompR genes in order to deregulate fatty acid uptake. To measure the effect of these deletions, copolymers were produced using recombinant E. coli and the repeating unit composition was analyzed. Residual fatty acid levels in media were also measured to determine a difference in ...


Sds Non-Acrylamide Polymeric Gel-Filled Capillary Electrophoresis For Molecular Size Separation Of Protein, Devon Andres Aug 1993

Sds Non-Acrylamide Polymeric Gel-Filled Capillary Electrophoresis For Molecular Size Separation Of Protein, Devon Andres

Honors Theses

Sodium dodecyl sulfide (SDS) non-acrylamide gel-filled capillary columns are a new technology being used for analysis and separation of biotechnology-derived proteins. This research was to compare this new technology to the current methods of SDS polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance size-exclusion chromatography (HPSEC). The molecular mass of four different recombinant proteins were determined by two commercialized SDS non-acrylamide gel-filled capillary columns, SDS-PAGE, and HPSEC. The data obtained showed that the SDS non-acrylamide gel-filled capillary columns were compatible with the SDS-PAGE technique for molecular mass determination. HPSEC was shown to be unreliable for molecular weight determination. SDS non-acrylamide gel-filled capillary ...