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FIU Electronic Theses and Dissertations

Hydrogen Bond Network

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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

The Influence Of The Proximal Thiolate Ligand And Hydrogen Bond Network Of The Proximal Helix On The Structural And Biochemical Properties Of Chloroperoxidase, Elena Shersher Mar 2016

The Influence Of The Proximal Thiolate Ligand And Hydrogen Bond Network Of The Proximal Helix On The Structural And Biochemical Properties Of Chloroperoxidase, Elena Shersher

FIU Electronic Theses and Dissertations

Chloroperoxidase (CPO) from Caldariomyces fumago is a versatile heme enzyme with great potential for environmental and pharmaceutical applications. It catalyzes a plethora of reactions including halogenation, dismutation, epoxidation, and oxidation. The diverse catalytic capabilities of CPO have long been attributed to the protein’s distinct active site that combines structural features of peroxidases and cytochromes P450. Particularly, the role of the axial thiolate ligand in CPO catalysis has been much debated. Furthermore, no data are available on the role of hydrogen bonding between Arg 26-Asn 37 and Ala 27-Asn 33 of the proximal helix in defining the structural and catalytic ...