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Biochemistry, Biophysics, and Structural Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Biotechnology

University of Nebraska - Lincoln

2006

Articles 1 - 2 of 2

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Purification And Initial Biochemical Characterization Of Atp:Cob(I)Alamin Adenosyltransferase (Eutt) Enzyme Of Salmonella Enterica, Nicole R. Baun, Jorge C. Escalante-Semerena Jan 2006

Purification And Initial Biochemical Characterization Of Atp:Cob(I)Alamin Adenosyltransferase (Eutt) Enzyme Of Salmonella Enterica, Nicole R. Baun, Jorge C. Escalante-Semerena

Biochemistry -- Faculty Publications

ATP:cob(I)alamin adenosyltransferase (EutT) of Salmonella

enterica was overproduced and enriched to ~70% homogeneity,

and its basic kinetic parameters were determined. Abundant

amounts of EutT protein were produced, but all of it remained

insoluble. Soluble active EutT protein (~70% homogeneous) was

obtained after treatment with detergent. Under conditions in which

cobalamin (Cbl) was saturating, Km(ATP) = 10 µM, kcat = 0.03 s-1,

and Vmax = 54.5 nM min-1. Similarly, under conditions in which

MgATPwas saturating,Km(Cbl) = 4.1µM, kcat = 0.06 s-1, andVmax=

105 nM min-1. Unlike ...


Sequential Processing Of A Mitochondrial Tandem Protein: Insights Into Protein Import In Schizosaccharomyces Pombe, Oleh Khalimonchuk, Martin Ott, Soledad Funes, Kai Ostermann, Gerhard Rödel, Johannes M. Herrmann Jan 2006

Sequential Processing Of A Mitochondrial Tandem Protein: Insights Into Protein Import In Schizosaccharomyces Pombe, Oleh Khalimonchuk, Martin Ott, Soledad Funes, Kai Ostermann, Gerhard Rödel, Johannes M. Herrmann

Biochemistry -- Faculty Publications

The sequencing of the genome of Schizosaccharomyces pombe revealed the presence of a number of genes encoding tandem proteins, some of which are mitochondrial components. One of these proteins (pre-Rsm22- Cox11) consists of a fusion of Rsm22, a component of the mitochondrial ribosome, and Cox11, a factor required for copper insertion into cytochrome oxidase. Since in Saccharomyces cerevisiae, Cox11 is physically attached to the mitochondrial ribosome, it was suggested that the tandem organization of Rsm22-Cox11 is used to covalently tie the mitochondrial ribosome to Cox11 in S. pombe. We report here that pre-Rsm22-Cox11 is matured in two subsequent processing events ...