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Biochemistry, Biophysics, and Structural Biology Commons

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Biotechnology

SUNY College of Environmental Science and Forestry

Articles 1 - 2 of 2

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Biochemical Conversion Of Biomass Into Butanol Using Clostridium Acetobutylicum, Eric Stevens Jan 2015

Biochemical Conversion Of Biomass Into Butanol Using Clostridium Acetobutylicum, Eric Stevens

Honors Theses

Butanol is a potential transportation fuel which could eventually replace fossil- derived petroleum and transition the world towards a more sustainable future. This fuel can be produced by enzymatically hydrolyzing pretreated lignocellulosic biomass, and simultaneously fermenting the resultant sugars with specific bacterial strains. In this experiment switchgrass was chemically pretreated in a 75% (v/v) ethanol and 1% H2SO4 (1% v/v) solution and subjected to simultaneous saccharification and fermentation (SSF) with hydrolytic enzymes and Clostridium acetobutylicum. The pH control method for the SSF apparatus was varied between a 50 mM acetate buffer solution (HOAc), and the addition of calcium ...


Deregulation Of Fatty Acid Transport In Escherichia Coli For Enhanced Control Of Biodegradable Plastic Copolymer Production, Ryan A. Scheel May 2014

Deregulation Of Fatty Acid Transport In Escherichia Coli For Enhanced Control Of Biodegradable Plastic Copolymer Production, Ryan A. Scheel

Honors Theses

Polyhydroxyalkanoates (PHA) are one possible alternative for petroleum-based plastics that have been shown to be produced effectively in bacterial systems utilizing fatty acids. The global regulatory genes arcA and ompR are known to regulate steps involved in fatty acid transport and metabolism, making them promising candidates for research. In this study, we deleted the arcA and ompR genes in order to deregulate fatty acid uptake. To measure the effect of these deletions, copolymers were produced using recombinant E. coli and the repeating unit composition was analyzed. Residual fatty acid levels in media were also measured to determine a difference in ...