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Biochemistry, Biophysics, and Structural Biology Commons

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Animal Sciences

Animal science

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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

A Candidate Gene Analysis For Response To Salmonella Enteritidis Challenge Or Vaccination In Young Chicks , Wei Liu Jan 2002

A Candidate Gene Analysis For Response To Salmonella Enteritidis Challenge Or Vaccination In Young Chicks , Wei Liu

Retrospective Theses and Dissertations

Salmonella enteritidis (SE) contamination of poultry products is a major cause of food-borne disease worldwide. The candidate gene approach for identification of quantitative trait loci (QTL) for chicken response to Salmonella enteritidis (SE) was applied in the current study. The Iowa Salmonella Response Resource Population (ISRRP), formed by crossing outbred broiler sires with three diverse, highly inbred dam lines (two major histocompatibility complex-congenic Leghorn lines G-B1 and G-B2 and one Fayoumi line) was studied. The F1 generation was evaluated as young chicks for either bacterial load in spleen and cecum after pathogenic SE inoculation, or antibody level after SE vaccination ...


Sequence Variation Characteristics Of D-Loop, Rrna, Trna, And Polypeptide Coding Region Of Bovine Mitochondrial Dna , Jianming Wu Jan 1995

Sequence Variation Characteristics Of D-Loop, Rrna, Trna, And Polypeptide Coding Region Of Bovine Mitochondrial Dna , Jianming Wu

Retrospective Theses and Dissertations

By cloning and sequencing the mtDNA of cows from different maternal lineages, it was found that multiple heteroplasmic mtDNA states within the D-loop and rRNA genes of mtDNA existed extensively in Holstein cattle. Meanwhile, the nature and transmission of mtDNA were investigated. The mtDNA population in a specific animal was found to be a mixture of different genotypes. Because heteroplasmy was observed frequently and seemingly is persistent, selected amplification of specific mtDNA molecules may not be a part of mtDNA inheritance. At same time, four hypervariable sites were located at nt 169, 216, and 1594 between nt 352 to 364 ...


The Relationship Between The Calpain Enzyme System And The Postmortem Degradation Of Selected Myofibrillar Proteins , Elisabeth Jane Huff Lonergan Jan 1995

The Relationship Between The Calpain Enzyme System And The Postmortem Degradation Of Selected Myofibrillar Proteins , Elisabeth Jane Huff Lonergan

Retrospective Theses and Dissertations

The protease [mu]-calpain degrades the myofibrillar proteins titin, nebulin, filamin, desmin and troponin-T. Titin and nebulin have been reported to be affected by sample preparation. This study showed that there was little difference in the rate of degradation of the intact forms due to sample preparation (whole muscle preparations vs. purified myofibrils). The postmortem degradation of titin, nebulin, filamin, desmin, and troponin-T was monitored using SDS-PAGE and Western blotting techniques at 0, 1, 3, 7, 14, 28, and 56 days postmortem. Postmortem aged samples with lower shear force values exhibited faster degradation of the five myofibrillar proteins. Similar degradation ...


Tissue-Specific Regulation Of 1,25-Dihydroxyvitamin D Action In The Target Tissues Of Rats , Matthew John Beckman Jan 1993

Tissue-Specific Regulation Of 1,25-Dihydroxyvitamin D Action In The Target Tissues Of Rats , Matthew John Beckman

Retrospective Theses and Dissertations

Tissue response to 1,25(OH)[subscript]2D[subscript]3 action was evaluated by examining vitamin D-dependent VDR, 24-OHase, and CaBP mRNA expressions, 24-OHase activities, and VDR concentrations in kidney and intestine. Of 4 groups of rats, 2 groups were fed an adequate vitamin D (300-600 IU/wk), 1.0-1.2% calcium (Ca) diet, designated NC, and 2 groups were fed an adequate vitamin D, 0.02% Ca diet, designated LC. One group from each diet was treated with excess vitamin D[subscript]3 (75,000 IU/wk). These groups were designated NCT and LCT, respectively;Plasma 1,25(OH ...