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Biochemistry, Biophysics, and Structural Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Animal Sciences

2000

Iowa State University

Articles 1 - 2 of 2

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Expression Of Bovine Mammary Gland Ca2+Atpases , Siriwan Prapong Jan 2000

Expression Of Bovine Mammary Gland Ca2+Atpases , Siriwan Prapong

Retrospective Theses and Dissertations

Control of transcellular Ca2+ fluxes associated with lactation are critical to mammary function and Ca2+ homeostasis. Mammary Ca2+ transport and storage may contribute to the pathological hypocalcemia seen in dairy cows at parturition. RT-PCR and Western Blotting were used to identify which Ca2+ ATPases are present in the bovine mammary gland and in milk fat globule membranes (MFGM). PMCA1, PMCA2 and PMCA4 are plasma membrane Ca2+ ATPases expressed in the bovine mammary gland. A putative Golgi Ca2+ ATPase (bSPCA) is also found in significant amounts in the mammary gland. The expression of Ca2+ ATPases in the bovine mammary gland is ...


Characterization Of [Alpha]-Adrenoceptor-Mediated Contractile Responses In The Isolated Bovine Tail Artery And Vein And An Investigation Of Nerve-Mediated Contraction In The Bovine Tail Artery In Response To Electrical Field Stimulation , Marina Val Ioudina Jan 2000

Characterization Of [Alpha]-Adrenoceptor-Mediated Contractile Responses In The Isolated Bovine Tail Artery And Vein And An Investigation Of Nerve-Mediated Contraction In The Bovine Tail Artery In Response To Electrical Field Stimulation , Marina Val Ioudina

Retrospective Theses and Dissertations

The purpose of this study was to identify alpha-adrenoreceptor types and subtypes mediating contractile responses to adrenergic agonists in the bovine tail artery and vein and to investigate nerve-mediated contraction in the bovine tail artery in response to electrical field stimulation. Isolated tissue bath methods were used to study isometric contractile responses in vessel rings. Electrical field stimulation, under constant current conditions, was used to study vessel contraction as a result of neurotransmitter(s) release from sympathetic nerve terminals;Evidence was obtained for the presence of functional alpha1A- and alpha1D-adrenoceptors in the tail artery. Whether functional alpha 1B-adrenoceptors are present ...