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Biochemistry, Biophysics, and Structural Biology Commons

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Articles 1 - 6 of 6

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Development Of An Enzyme-Linked Immunosorbent Assay (Elisa) For Quantification Of Skeletal Muscle Calpastatin, M. E. Doumit, Steven M. Lonergan, J. R. Arbona, J. Killefer, M. Koohmaraie Jan 1996

Development Of An Enzyme-Linked Immunosorbent Assay (Elisa) For Quantification Of Skeletal Muscle Calpastatin, M. E. Doumit, Steven M. Lonergan, J. R. Arbona, J. Killefer, M. Koohmaraie

Steven M. Lonergan

An indirect antibody ELISA was developed for rapid and sensitive quantification of skeletal muscle calpastatin. Polyclonal antibodies were raised in rabbits against recombinant calpastatin, corresponding to domains 2, 3, and 4 of bovine skeletal muscle calpastatin. Western blot analysis revealed that these antibodies specifically recognize an immunoreactive calpastatin protein of approximately 130 kDa in prerigor skeletal muscle extracts. The intensity of the immunoreactive bands corresponds qualitatively with assayable calpastatin activity. For ELISA development, optimum dilutions of sample, primary anti-calpastatin antibody, and peroxidase-conjugated secondary antibody were determined by titration. A dilution optimum for coating of Immulonâ 4 (Dynatech) plates was observed ...


The Role Of Rat Brain Ptp-1 Surface Basic Residues In Substrate Reactivity , Deborah Sue Baedke Jan 1996

The Role Of Rat Brain Ptp-1 Surface Basic Residues In Substrate Reactivity , Deborah Sue Baedke

Retrospective Theses and Dissertations

Previous work by others has demonstrated that rat brain protein tyrosine phosphatase (rbPTP-1) exhibited increased reactivity toward substrates containing acidic residues N-terminal to phosphotyrosine. We show that substrates containing a single acidic residue C-terminal to the phosphotyrosine also enhanced reactivity with rbPTP-1. A substrate aspartate residue at the P+2 position exhibited the greatest enhancement of reactivity;Surface basic residues of rbPTP-1 were investigated for their role in the increased reactivity due to substrate acidic residues. Mutation of R47 or K120 to alanine decreased the enhancement of reactivity due to acidic residues N-terminal to phosphotyrosine by 78% and 73% respectively ...


Natural History Note: Elaphe Obsoleta Obsoleta (Black Rat Snake) - Foraging, James Withgott, Charles Amlaner Dec 1995

Natural History Note: Elaphe Obsoleta Obsoleta (Black Rat Snake) - Foraging, James Withgott, Charles Amlaner

Charles J. Amlaner

No abstract provided.


Monitoring Body Temperature And Nocturnal Activity Of Snakes With Implanted Transmitters, James Withgott, Charles Amlaner Dec 1995

Monitoring Body Temperature And Nocturnal Activity Of Snakes With Implanted Transmitters, James Withgott, Charles Amlaner

Charles J. Amlaner

No abstract provided.


Natural History Note: Elaphe Obsoleta Obsoleta (Black Rat Snake) - Response To Fire, James Withgott, Charles Amlaner Dec 1995

Natural History Note: Elaphe Obsoleta Obsoleta (Black Rat Snake) - Response To Fire, James Withgott, Charles Amlaner

Charles J. Amlaner

No abstract provided.


Transmitter Implantation Techniques In Black Rat Snakes, Charles Amlaner, James Withgott Dec 1995

Transmitter Implantation Techniques In Black Rat Snakes, Charles Amlaner, James Withgott

Charles J. Amlaner

No abstract provided.