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Articles 1 - 5 of 5

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Monovalent Cation Induced Structural Transitions In Telomeric Dnas: G-Dna Folding Intermediates, Charles C. Hardin, Eric Henderson, Thomas Watson, Joyce K. Prosser May 1991

Monovalent Cation Induced Structural Transitions In Telomeric Dnas: G-Dna Folding Intermediates, Charles C. Hardin, Eric Henderson, Thomas Watson, Joyce K. Prosser

Zoology and Genetics Publications

Telomeric DNA consists of G- and C-rich strands that are always polarized such that the G-rich strand extends past the 3’ end of the duplex to form a 12-16-base overhang. These overhanging strands can self-associate in vitro to form intramolecular structures that have several unusual physical properties and at least one common feature, the presence of non-Watson-Crick G-G base pairs. The term “G-DNA” was coined for this class of structures (Cech, 1988). On the basis of gel electrophoresis, imino proton NMR, and circular dichroism (CD) results, we find that changing the counterions from sodium to potassium (in 20 mM phosphate ...


Purification And Properties Of The Malic Enzyme And The Malate Dehydrogenases From The Gill Tissue Of The Ribbed Mussel (Modiolus Demissus) , Mary Michael Brodey Jan 1991

Purification And Properties Of The Malic Enzyme And The Malate Dehydrogenases From The Gill Tissue Of The Ribbed Mussel (Modiolus Demissus) , Mary Michael Brodey

Retrospective Theses and Dissertations

To assess the role of dicarboxylic acid metabolism in the synthesis of alanine and other compounds by the ribbed mussel under conditions of hyperosmotic and anaerobic stress, the cytosolic and mitochondrial malate dehydrogenases and the mitochondrial malic enzyme from this organism were purified and their kinetics studied. Both the cytosolic and mitochondrial malate dehydrogenases were found to be dimers with native M[subscript] r = 60,000. One cMDH allele (designated slow) was found to predominate in populations from Cape Cod, MA. Similar low K[subscript] m's were observed for purified preparations of the slow/slow homodimer (cMDH) and for ...


Avena Phytochrome Mrna: In Vivo Localization, Degradation Products, And Half-Life , Kevin Andrew Seeley Jan 1991

Avena Phytochrome Mrna: In Vivo Localization, Degradation Products, And Half-Life , Kevin Andrew Seeley

Retrospective Theses and Dissertations

The purpose of this research is to determine the role that post-transcriptional processes play in the regulation of phytochrome (phy) steady-state messenger RNA (mRNA) levels. The control of etiolated phytochrome (phyA) and chlorophyll a/b binding protein (cab) gene transcription and steady-state mRNA levels are known to be regulated by the phyA signal transduction pathway. I performed a number of control experiments that indicate the degradation products detected in northern analysis of phyA mRNA were produced in vivo and were not an artifact of RNA isolation techniques. I have also shown that infrared (IR) irradiation has no appreciable effect on ...


Biotelemetry Xi: Proceedings Of The Eleventh International Symposium On Biotelemetry, Charles Amlaner (Editor), Akihiko Uchiyama (Editor) Dec 1990

Biotelemetry Xi: Proceedings Of The Eleventh International Symposium On Biotelemetry, Charles Amlaner (Editor), Akihiko Uchiyama (Editor)

Charles J. Amlaner

Proceedings of the Eleventh International Symposium on Biotelemetry, held in Yokohama, Japan, on August 29 - September 4, 1990.


Biotelemetry And Radio Tracking: A Tutorial Review, Charles Amlaner Dec 1990

Biotelemetry And Radio Tracking: A Tutorial Review, Charles Amlaner

Charles J. Amlaner

No abstract provided.