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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Irreversible Thiol Oxidation In Carbonic Anhydrase Iii: Protection By S-Glutathiolation And Detection In Aging Rats, Robert J. Mallis, Michael J. Hamann, Wei Zhao, Tiequan Zhang, Suzanne Hendrich, James A. Thomas Mar 2002

Irreversible Thiol Oxidation In Carbonic Anhydrase Iii: Protection By S-Glutathiolation And Detection In Aging Rats, Robert J. Mallis, Michael J. Hamann, Wei Zhao, Tiequan Zhang, Suzanne Hendrich, James A. Thomas

Food Science and Human Nutrition Publications

Proteins with reactive sulfhydryls are central to many important metabolic reactions and also contribute to a variety of signal transduction systems. In this re- port, we examine the mechanisms of oxidative dam- age to the two reactive sulfhydryls of carbonic anhy- drase III. Hydrogen peroxide (H2O2), peroxy radicals, or hypochlorous acid (HOCl) produced irreversibly oxidized forms, primarily cysteine sulfinic acid or cys- teic acid, of carbonic anhydrase III if glutathione (GSH) was not present. When GSH was approximate- ly equimolar to protein thiols, irreversible oxidation was prevented. H2O2 and peroxyl radicals both gener- ated S-glutathiolated carbonic anhydrase III via par- ...