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Biochemistry, Biophysics, and Structural Biology Commons

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Animal Sciences

Food Science and Human Nutrition Publications

2013

Articles 1 - 1 of 1

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Analysis Of Resistant Starches In Rat Cecal Contents Using Fourier Transform Infrared Photoacoustic Spectroscopy, Timothy J. Anderson, Yongfeng Ai, Roger W. Jones, Robert S. Houk, Jay-Lin Jane, Yinsheng Zhao, Diane F. Birt, John F. Mcclelland Jan 2013

Analysis Of Resistant Starches In Rat Cecal Contents Using Fourier Transform Infrared Photoacoustic Spectroscopy, Timothy J. Anderson, Yongfeng Ai, Roger W. Jones, Robert S. Houk, Jay-Lin Jane, Yinsheng Zhao, Diane F. Birt, John F. Mcclelland

Food Science and Human Nutrition Publications

Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) qualitatively and quantitatively measured resistant starch (RS) in rat cecal contents. Fisher 344 rats were fed diets of 55% (w/w, dry basis) starch for 8 weeks. Cecal contents were collected from sacrificed rats. A corn starch control was compared against three RS diets. The RS diets were high-amylose corn starch (HA7), HA7 chemically modified with octenyl succinic anhydride, and stearic-acid-complexed HA7 starch. To calibrate the FTIR-PAS analysis, samples from each diet were analyzed using an enzymatic assay. A partial least-squares cross-validation plot generated from the enzymatic assay and FTIR-PAS spectral results for starch ...