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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

The Study Of Alcoholic Liver Diseases, Seun Emmanuel Owoseni Dec 2018

The Study Of Alcoholic Liver Diseases, Seun Emmanuel Owoseni

Electronic Theses and Dissertations

Excessive alcohol consumption is the primary contributing factor in the development of alcoholic liver diseases (ALD). Nicotine contained in tobacco is a major addictive alkaloid, which enhances the effects of ALDs. The major enzyme involved in nicotine metabolism is cytochrome P450 2A5 (CYP2A5) which is produced in the liver. Alcohol can stimulate the CYP2A5 enzyme. We utilized cyp2a5-/- knockout mice in this research to examine the effects of CYP2A5.

The cyp2a5-/- mice and wild-type (WT) mice were fed liquid ethanol diet with or without nicotine to induce ALD. Nicotine enhancing effects on ALD were observed in WT mice but not ...


Temperature And Polyunsaturated Fatty Acid’S Effect On Daphnia Magna Reproduction, Mark Albright Dec 2018

Temperature And Polyunsaturated Fatty Acid’S Effect On Daphnia Magna Reproduction, Mark Albright

Electronic Theses and Dissertations

Organisms adapt to their environments by adjusting their biochemistry and physiology; such adaptation is limited by resource availability and physiological constraints. The freshwater crustacean Daphnia magna inhabits a wide range of environments and must survive and reproduce within a range of temperatures. One limit to low-temperature adaptation is thought to be the availability of unsaturated fatty acids necessary to maintain proper fluidity of cellular membranes. D. magna maintained at 10 ºC on a diet poor in unsaturated fatty acids have been observed to produce clutches that fail to develop. However, this has not been observed on a diet rich in ...


Evaluation Of Different Probiotic Strains Supplemented In Commercial Broiler Rations And Their Influences On Performance, Yield, And Intestinal Microbiota., Justin M. Glasscock May 2017

Evaluation Of Different Probiotic Strains Supplemented In Commercial Broiler Rations And Their Influences On Performance, Yield, And Intestinal Microbiota., Justin M. Glasscock

Electronic Theses and Dissertations

The objective of this study was to evaluate Bacillus megaterium, Bacillus licheniformis, and Bacillus subtilis, as probiotic strains compared to Bacitracin Methylene Disalicylate (BMD) 50 as the antibiotic growth promotor, supplemented in commercial broiler rations and their influences on performance, yield, and intestinal microbiota. This trial was completed as a randomized-block design with 4,800 birds split into 96, 5’x10’ pens, and randomly assigned to one of eight treatment groups. The birds were placed at a stocking density of 1.00 ft2/bird, (50 birds/pen), and reared on used pine shaving for 55 days. Throughout the study ...


Exploring The Relationship Between Behaviour And Neurochemistry In The Polyphenic Spider, Anelosimus Studiosus (Araneae: Theridiidae), Jennifer B. Price Aug 2016

Exploring The Relationship Between Behaviour And Neurochemistry In The Polyphenic Spider, Anelosimus Studiosus (Araneae: Theridiidae), Jennifer B. Price

Electronic Theses and Dissertations

The importance of social behaviour is evident in human society, but there are both costs and benefits associated with cooperation and sociality throughout the animal kingdom. At what point do the benefits outweigh the costs, and when do selective pressures favour sociality and colonization over solitude and independence? To investigate these questions, we have focused on an anomalous species of spider, Anelosimus studiosus, also known now as the northern social spider. Throughout its broad range, A. studiosus is solitary and aggressive, but recently, colonies of cooperative and social individuals have been observed at northern latitudes. This leads to two research ...


Vaccines For Infection Salmon Anemia Virus, Nathan Edward Charles Brown May 2003

Vaccines For Infection Salmon Anemia Virus, Nathan Edward Charles Brown

Electronic Theses and Dissertations

Infectious salmon anemia (ISA) virus is an emerging pathogen of fanned Atlantic salmon. Due to the massive economic losses inflicted by the ISA virus, effective measures to control future outbreaks are necessary. An attractive method for preventing ISA virus from infecting stocks of Atlantic salmon is vaccination. DNA vaccination is a proven cheap, effective means of protecting fish from aquatic viruses. In this thesis DNA vaccination of Atlantic salmon was investigated. Three different strains of ISA virus were cultured and purified by density gradient ultracentrifugation. ISA virus RNA was isolated and used to amplify full-length cDNAs of five ISA virus ...


Cloning Of Bovine Placental Lactogen And Production In Vitro, Stephanie A. Doucette Jan 2003

Cloning Of Bovine Placental Lactogen And Production In Vitro, Stephanie A. Doucette

Electronic Theses and Dissertations

Bovine placental lactogen (bPL) is a hormone produced by the fetal portion of the placenta during gestation. Although there is little known about the structure and function of the protein, it is thought to play a role in the growth of the fetus and in mammary growth and differentiation. Attempts have been made to heighten the understanding of bPL through the use of recombinant DNA technology. Thus far, researchers have been able to produce the recombinant molecule in bacteria cells. Bacteria, however, are incapable of carrying out some of the post-translational modifications characteristic of mammalian proteins. For example, unlike native ...