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Zoology and Genetics Publications

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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Atomic Force Microscopy And Manipulation Of Living Glial Cells, V. Parpura, P. G. Haydon, D. S. Sakaguchi, E. Henderson Jan 1993

Atomic Force Microscopy And Manipulation Of Living Glial Cells, V. Parpura, P. G. Haydon, D. S. Sakaguchi, E. Henderson

Zoology and Genetics Publications

The atomic force microscope (AFM) is capable of imaging surfaces at very high resolution. The AFM has been used to image living glial cells in culture. Typical images reveal the three‐dimensional shape of the cell and often internal cellular structures are visible. In this report, it is shown that by increasing the imaging force, cells can be removed from the surface on which they are grown. Although the forces involved in this process are complex, it is possible to compare relative adhesion of different types of living cells to a particular substrate.


Monovalent Cation Induced Structural Transitions In Telomeric Dnas: G-Dna Folding Intermediates, Charles C. Hardin, Eric Henderson, Thomas Watson, Joyce K. Prosser May 1991

Monovalent Cation Induced Structural Transitions In Telomeric Dnas: G-Dna Folding Intermediates, Charles C. Hardin, Eric Henderson, Thomas Watson, Joyce K. Prosser

Zoology and Genetics Publications

Telomeric DNA consists of G- and C-rich strands that are always polarized such that the G-rich strand extends past the 3’ end of the duplex to form a 12-16-base overhang. These overhanging strands can self-associate in vitro to form intramolecular structures that have several unusual physical properties and at least one common feature, the presence of non-Watson-Crick G-G base pairs. The term “G-DNA” was coined for this class of structures (Cech, 1988). On the basis of gel electrophoresis, imino proton NMR, and circular dichroism (CD) results, we find that changing the counterions from sodium to potassium (in 20 mM phosphate ...