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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
The Evolution Of Platelet Responses To Collagen Under Conditions Of Hemodynamic Flow, Alec A. Schmaier
Publicly Accessible Penn Dissertations
Collagen activates platelets through the immune-type GPVI receptor, but the mechanism by which this receptor has adapted to function in platelets under conditions of rapid blood flow is unclear. Additionally, it is unknown if responses to collagen are conserved in nucleated thrombocytes found in non-mammalian vertebrates, which do not express GPVI. Here we show that the GPVI proline-rich domain accelerates GPVI signaling kinetics and is required for maximal adhesion to collagen under flow. The proline-rich domain accelerates signaling through binding and directly activating the Src-family kinase Lyn. Chicken thrombocytes are potently activated by collagen but do not form 3-dimensional aggregates ...