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Articles 1 - 11 of 11

Full-Text Articles in Life Sciences

Book Review Of "Of Flies, Mice, And Men: On The Revolution In Molecular Biology, By One Of The Scientists Who Helped Make It" By François Jacob, Nancy Curtis Dec 1998

Book Review Of "Of Flies, Mice, And Men: On The Revolution In Molecular Biology, By One Of The Scientists Who Helped Make It" By François Jacob, Nancy Curtis

Library Staff Publications

No abstract provided.


Testosterone Effects On Renal Norepinephrine Content And Release In Rats With Different Y Chromosomes, Thomas J. Jones, Gail Dunphy, Amy Milsted, Daniel Ely Nov 1998

Testosterone Effects On Renal Norepinephrine Content And Release In Rats With Different Y Chromosomes, Thomas J. Jones, Gail Dunphy, Amy Milsted, Daniel Ely

Biology Faculty Research

The Y chromosome in spontaneously hypertensive rats (SHR) and stroke-prone rats has been shown to contain a locus that contributes to the hypertensive effect; both the sympathetic nervous system and testosterone may be involved. The objective of this study was to look at the effects of testosterone on renal norepinephrine (NE) release and content in the isolated perfused kidney in different Y chromosome backgrounds. The study involved male SHR, Wistar-Kyoto rats (WKY), and 2 consomic strains with different Y chromosomes (n=5 to 8 per group). Adult animals were castrated, and implants containing testosterone propionate were placed at the base ...


Analytical Method Using Modified Scanning Probes, Eric R. Henderson, Curtis L. Mosher, Vivian Wynne Jones, John-Bruce D. Green, Marc D. Porter Jun 1998

Analytical Method Using Modified Scanning Probes, Eric R. Henderson, Curtis L. Mosher, Vivian Wynne Jones, John-Bruce D. Green, Marc D. Porter

Iowa State University Patents

The present invention provides a method of analyzing for a specific material in a sample using a sensor including a resonating member having resonating properties. The resonating member has a probe and a known material is disposed on or forms the probe. The method includes the steps of positioning the sensor proximate to the sample, detecting a force dependent change in the resonance properties of the sensor, and confirming the presence of the specific material based on the identity of the known material and the detection of a resonance change.


Heritability Of Expression Of The 70kd Heat-Shock Protein In Drosophila Melanogaster And Its Relevance To The Evolution Of Thermotolerance, Robert A. Krebs, Martin E. Feder, Jeehyun Lee Jun 1998

Heritability Of Expression Of The 70kd Heat-Shock Protein In Drosophila Melanogaster And Its Relevance To The Evolution Of Thermotolerance, Robert A. Krebs, Martin E. Feder, Jeehyun Lee

Biological, Geological, and Environmental Faculty Publications

The principle inducible heat-shock protein of Drosophila melanogasrer, Hsp70, contributes to thermotolerance throughout the entire life cycle of the species but may also reduce fitness in some life stages. In principle, selection might maximize the benefits of Hsp70 expression relative to its costs by adjusting the magnitude of Hsp70 expression for each life-cycle stage independently. Therefore we examined whether the magnitude of Hsp70 ex pre sion varied during the life cycle and the relationship of this variation to several life-history traits. For 28 isofemale lines derived from a single natural population, estimates of heritable variation in Hsp70 expression ranged between ...


Family With Bannayan-Riley-Ruvalcaba Syndrome: Linkage..., J. Adam Jelinek Apr 1998

Family With Bannayan-Riley-Ruvalcaba Syndrome: Linkage..., J. Adam Jelinek

Life and Environmental Sciences Undergraduate Theses

The purpose of this study was to locate the gene responsible for Bannayan-Riley-Ruvalcaba syndrome in an affected family. DNA was isolated from 13 members of the family and linkage studies were conducted. Twelve markers encompassing a specific region on chromosome 10 (10q23) were used in linkage analysis. DNA segments specific for each primer set were amplified using PCR (polymerase chain reaction) techniques and then analysis of the products was conducted using the ABI genetic analyzer. Alleles were then assigned to each family member and linkage was tested (LINKAGE 5.0). A maximum LOD score of 0.98 was obtained at ...


Intracellular Coexpression Of Epidermal Growth Factor Receptor, Her-2/Neu, And P21ras In Human Breast Cancers: Evidence For The Existence Of Distinctive Patterns Of Genetic Evolution That Are Common To Tumors From Different Patients, Stanley E. Shackney, Agnese A. Pollice, Charles A. Smith, Laura E. Janocko, Lillian Sweeney, Kathryn A. Brown, Sarita G. Singh, Lingping Gu, Robert Yakulis, Joseph F. Lucke Jan 1998

Intracellular Coexpression Of Epidermal Growth Factor Receptor, Her-2/Neu, And P21ras In Human Breast Cancers: Evidence For The Existence Of Distinctive Patterns Of Genetic Evolution That Are Common To Tumors From Different Patients, Stanley E. Shackney, Agnese A. Pollice, Charles A. Smith, Laura E. Janocko, Lillian Sweeney, Kathryn A. Brown, Sarita G. Singh, Lingping Gu, Robert Yakulis, Joseph F. Lucke

Joseph Lucke

Multiparameter flow cytometry studies were performed on cells from the primary tumors of 94 patients with breast cancer. Correlated cellular measurements of cell DNA content, Her-2/neu, epidermal growth factor receptor (EGFR), and p21ras levels were performed on each of 5,000 to 100,000 cells from each tumor. When criteria for positivity were matched with those in common use for immunohistochemical studies, 28 of 94 (30\%) breast cancers were classified as positive for Her-2/neu overexpression. When similar criteria were applied to the EGFR measurements, 23 of 94 (24\%) cases were classified as positive for EGFR overexpression. Similarly, 23 ...


Isolation And Characterization Of Genes Belonging To Developmental Regulatory Gene Classes In Potato (Solanum Tuberosum L.) , Jennifer Krulic Hart Jan 1998

Isolation And Characterization Of Genes Belonging To Developmental Regulatory Gene Classes In Potato (Solanum Tuberosum L.) , Jennifer Krulic Hart

Retrospective Theses and Dissertations

Although plant growth is an important topic, the regulation of genes involved in growth is poorly understood. To contribute to current information about plant growth regulation, an early tuberization cDNA library from Solanum tuberosum L., expected to contain genes involved in cell proliferation, was screened for members of developmental regulatory gene families. The two objectives of this study were to isolate genes that might be involved in plant growth regulation, and to characterize the RNA accumulation patterns of these genes to gain insight into their function. The AAA-protein superfamily ATPase POTATP1 and the homeobox gene POTH1 were isolated to accomplish ...


Characterization Of Starch Debranching Enzymes Of Maize Endosperm , Afroza Rahman Jan 1998

Characterization Of Starch Debranching Enzymes Of Maize Endosperm , Afroza Rahman

Retrospective Theses and Dissertations

The goal of this research is to understand the biochemical lesion of the sugary1 mutation in maize. The sugary1 gene is a determinant of starch structure. Homozygous su1-mutant endosperms accumulate a highly-branched water-soluble polysaccharide, phytoglycogen, at the expense of amylopectin. These suggest that both branched polysaccharides share a common biosynthetic pathway, and that SU1 participates in the biosynthesis of kernel starch. Specific antibodies were used to demonstrate that SU1 is a 79-kD protein that accumulates in endosperm during starch biosynthesis. Recombinant near full-length SU1 (SU1r) was expressed in E. coli and purified to apparent homogeneity. Determination of substrate specificity of ...


Characterization Of Three Root Hair Mutants In Maize , Tsui-Jung Wen Jan 1998

Characterization Of Three Root Hair Mutants In Maize , Tsui-Jung Wen

Retrospective Theses and Dissertations

Root hairs are thought to play an important role in the uptake of water and nutrients from the environment. Several root hair defective mutants were isolated from Mutator-tagged stocks and EMS-mutagenized lines of maize. These mutants all condition either non-elongated root-hair initials (rth1, rth3-1, rth3-2, rth3-3, rth4, rth5, rth6) or shorter-than-normal root hairs (rth2). The morphology of rth1, rth2, and rth3 root hairs were analyzed via SEM. The rth1, rth2, and rth3 genes map to chromosomes 1L, 5L, and 1S, respectively. The rth3 gene was cloned using Mu-tagged allele. The partial cDNA sequence of this gene suggests that it may ...


Physiological Consequences Of Astrocyte-To-Neuron Signaling , Rita Prabhakar Sanzgiri Jan 1998

Physiological Consequences Of Astrocyte-To-Neuron Signaling , Rita Prabhakar Sanzgiri

Retrospective Theses and Dissertations

The traditional view of the central nervous system (CNS) in which only neurons are capable of active intercellular signaling while glial cells merely provide structural and trophic support to neurons, has been challenged by a number of new findings. Recent evidence shows that astrocytes, a subtype of glial cells, participate in inter-astrocyte signaling by using variations in their cytosolic Ca2+ levels (Cornell-Beil et al., 1990; Charles et al., 1991; Finkbeiner et al., 1992; Duffy and MacVicar, 1995). Neuronal activity can trigger these Ca2+ increases in astrocytes indicating that neurons can signal to astrocytes (Dani et al., 1992; Porter and McCarthy ...


Characterization Of The Blackpatch Mutation In Drosophila Melanogaster , Mythreyi D. Shastri Jan 1998

Characterization Of The Blackpatch Mutation In Drosophila Melanogaster , Mythreyi D. Shastri

Retrospective Theses and Dissertations

Mutations at the Blackpatch locus cause death and degeneration in the developing visual system of Drosophila melanogaster. Some Bpt alleles cause recessive lethality in the larval stage during development. To understand the role of Bpt in visual system development, the mutant phenotype was characterized for its effects on structure of the developing eye and the electrical activity of adult mutant eyes. The first hypothesis was that Bpt gene function may be required for the visual transduction pathway to receive and process light signals correctly. This is an electrical/biochemical process that can be visualised as an electroretinogram (ERG) and mutations ...